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Simultaneous Analysis of Macamides in Maca (Lepidium meyenii) with Different Drying Process by Liquid Chromatography Tandem Mass Spectrometry

文献类型： 外文期刊

作者： Pan, Yu 1 ; Zhang, Ji 1 ; Li, Hong 1 ; Wang, Yuan-Zhong 1 ; Li, Wan-Yi 1 ;

作者机构： 1.Yunnan Acad Agr Sci, Inst Med Plants, 2238 Beijing Rd, Beijing 2238, Peoples R China

2.Yunnan Tech Ctr Qual Chinese Mat Med, Kunming 650200, Peoples R China

关键词： Maca;Macamides;Drying process;UPLC-MS/MS

期刊名称：FOOD ANALYTICAL METHODS （ 影响因子：3.366； 五年影响因子：3.07 ）

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收录情况： SCI

摘要： Macamides with benzylalkylamides structure are a group of characteristic constituents isolated from functional food Maca (Lepidium meyenii Walp.). Previous study demonstrated that macamides are secondary amides, the accumulation of which is associated with drying process. In this study, a rapid method based on ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) is developed for the simultaneous detection and determination of macamides in Maca. Validation parameters including linearity, limit of detection, limit of quantification, accuracy, precision, repeatability, and stability were all within the required limits. Seven major macamides are determined by the UPLC method with detection wavelength at 205 nm in 12 min. Based on the fragmentation pattern of standard compounds, four fragment ions (m/z 91, 108, 121, and 138) are selected as diagnostic ions for detection of macamides through precursor ion acquisition mode. Additionally, comparative analysis is performed to examine the effects of different drying processes (air-drying, oven-drying, freeze-drying, and steaming) on Maca (whole hypocotyls and pieces) for macamides. Whole hypocotyls with oven-drying showed the highest content of macamides (2.528 mg/g), while freeze-drying and steaming significantly decrease the amount of macamides. The results provide a reliable method for analysis of macamides and extend previous work insights into the drying process of Maca.

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[1]Characteristic fingerprinting based on macamides for discrimination of maca (Lepidium meyenii) by LC/MS/MS and multivariate statistical analysis. Pan, Yu,Zhang, Ji,Li, Hong,Wang, Yuan-Zhong,Li, Wan-Yi,Pan, Yu,Zhang, Ji,Li, Hong,Wang, Yuan-Zhong,Li, Wan-Yi.

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