CHARACTERIZATION OF CYMBIDIUM CHLOROTIC MOSAIC VIRUS FROM CYMBIDIUM TORTISEPALUM IN CHINA
文献类型： 外文期刊
作者： Zhang, Z. 2 ; Zheng, K. 2 ; Dong, J. 2 ; McBeath, J. H. 3 ; Wang, X. 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China
2.Yunnan Acad Agr Sci, Inst Biotechnol & Germplasm Resources, Key Lab Southwestern Crop Gene Resources & Germpl, Yunnan Prov Key Lab Agr Biotechnol,Minist Agr, Kunming, Yunnan, Peoples R China
3.Univ Alaska Fairbanks, Plant Pathol & Biotechnol Lab, Fairbanks, AK USA
关键词： Sobemovirus;electron microscopy;deep sequencing
期刊名称：JOURNAL OF PLANT PATHOLOGY （ 影响因子：1.729； 五年影响因子：1.681 ）
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收录情况： SCI
摘要： The complete nucleotide sequence of isolate YN of the putative sobemovirus Cymbidium chlorotic mosaic virus (CyCMV) from Cymbidium tortisepalum plants showing chlorotic and necrotic spots in Yunnan province (southwest China) was determined by deep sequencing. Dips from symptomatic leaves showed that this virus has icosahedral particles ca. 30 nm in diameter. The viral genome is a positive-strand RNA molecule 4,081 nt in size (GenBank accession No. KR996515) encoding four open reading frames with an organization similar to that described for sobemoviruses. The complete sequence of CyCMV-YN shares 89.5% identity at the nucleotide level with that of CyCMV-JP isolated from Cymbidium goeringii in Japan. This is the first report of a sobemovirus infecting orchid in China.
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