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Fungal elicitors enhance ginsenosides biosynthesis, expression of functional genes as well as signal molecules accumulation in adventitious roots of Panax ginseng C. A. Mey

文献类型： 外文期刊

作者： Li, Jinxin 1 ; Liu, Shujie 2 ; Wang, Juan 1 ; Li, Jing 1 ; Liu, Dahui 3 ; Li, Jianli 1 ; Gao, Wenyuan 1 ;

作者机构： 1.Tianjin Univ, Sch Pharmaceut Sci & Technol, Tianjin Key Lab Modern Drug Delivery & High Effic, Tianjin 300072, Peoples R China

2.Tianjin Univ, Key Lab Syst Bioengn, Minist Educ, Tianjin 300072, Peoples R China

3.Yunnan Acad Agr Sci, Inst Med Plants, Kunming 65023, Yunnan, Peoples R China

关键词： Adventitious root;Fungal elicitor;HPLC-ESI-MSn;Biosynthetic genes;Signal molecules

期刊名称：JOURNAL OF BIOTECHNOLOGY （ 影响因子：3.307； 五年影响因子：3.778 ）

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收录情况： SCI

摘要： In this work, we selected three fungi strains (Aspergillus niger, Aspergillus flavus and Aspergillus oryzae) as elicitors prepared from mycelium or fermentation broth to improve ginsenosides production in adventitious roots culture. The results showed that ginsenosides production (29.90 +/- 4,67 mg g(-1)) was significantly enhanced upon elicitation with 200 mg L-1 A. niger elicitor prepared from mycelium, which was 3.52-fold of untreated group. HPLC-ESI-MPn analysis was performed, showing that ginsenoside Rb-3 was present after treatment with the A. niger. Furthermore, we found that A. niger significantly enhanced accumulation of Nitric oxide (NO), salicylic acid (SA) and jasmonic acid (JA) involved in plant defense response, and significantly up-regulated the expression of the geranyl diphosphate synthase (GPS), farnesyl diphosphate synthase (FPS), squalene synthase (SS), squalene epoxidase (SE), dammarenediol synthase (DS), Two cytochrome P450 (CYP) genes (CYP716A47 and CYP716A53v2) and three UDP-glycosyltransferases (UGT) genes (UGTAE2, UGT94Q2 and UGTpg100). (C) 2016 Elsevier B.V. All rights reserved.

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[1]Aspergillus niger Enhance Bioactive Compounds Biosynthesis As Well As Expression of Functional Genes in Adventitious Roots of Glycyrrhiza uralensis Fisch. Li, Jing,Wang, Juan,Li, Jinxin,Li, Hongfa,Gao, Wenyuan,Li, Jing,Liu, Dahui,Li, Jianli,Liu, Shujie.

[2]Analysis of ginsenoside content, functional genes involved in ginsenosides biosynthesis, and activities of antioxidant enzymes in Panax quinquefolium L. adventitious roots by fungal elicitors. Li, Jinxin,Li, Hongfa,Wang, Juan,Liu, Shujie,Li, Jianli,Liu, Dahui.

[3]iTRAQ Protein Profiling of Adventitious Root Formation in Mulberry Hardwood Cuttings. Tang, Zhuang,Du, Wei,Du, XiaoLong,Ban, YueYuan,Cheng, JiaLing,Du, Wei,Cheng, JiaLing.

[4]Comprehensive Analysis of R2R3-MYB Genes During Adventitious Root Formation in Cuttings of Morus alba. Du, Xiao Long,Cao, Xu,Yin, Chao Rui,Tang, Zhuang,Ban, Yue Yuan,Cheng, Jia Ling,Du, Wei.

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