Quantitative evaluation and discrimination of wild Paris polyphylla var. yunnanensis (Franch.) Hand.-Mazz from three regions of Yunnan Province using UHPLC-UV-MS and UV spectroscopy couple with partial least squares discriminant analysis
文献类型： 外文期刊
作者： Yang, Yuangui 1 ; Jin, Hang 2 ; Zhang, Ji 2 ; Zhang, Jinyu 1 ; Wang, Yuanzhong 1 ;
作者机构： 1.Yunnan Univ Tradit Chinese Med, Coll Tradit Chinese Med, Kunming 650500, Peoples R China
2.Yunnan Acad Agr Sci, Inst Med Plants, Kunming 650200, Peoples R China
3.Yunnan Tech Ctr Qual Chinese Mat Med, Kunming 650200, Peoples R China
关键词： Paris polyphylla var. yunnanensis (Franch.) Hand.-Mazz;UHPLC-UV-MS;UV;PLS-DA
期刊名称：JOURNAL OF NATURAL MEDICINES （ 影响因子：2.343； 五年影响因子：2.301 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要：
Paris polyphylla var. yunnanensis (Franch.) Hand.-Mazz (PPY) is used widely as an anthelmintic, antimicrobial, and anti-tumor agent. Multiplicate analytical methods have been employed to discriminate PPY from different regions, as well as to identify regions most beneficial to the growing of this species. In this study, a convenient and accurate method was established using ultra high performance liquid chromatography (UHPLC) for simultaneous determination of four steroid saponins (Pa, Pb, polyphyllin VI, and chonglou saponin VII). Partial least squares discriminant analysis (PLS-DA) according to UHPLC and UV spectroscopy was applied to analyze 30 samples of PPY from three regions of Yunnan Province in China, and identify significant peaks. The results indicated that the correlation coefficients (r (2)) of all calibration curves were above 0.999, and the inter- and intra-day relative standard deviations (RSD) of retention time and peak areas of common peaks were below 1.78 % and 3.40 %, respectively, with recovery rates of 99.6-103.4 % with RSD ae
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