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A protease-resistant alpha-galactosidase from Pleurotus djamor with broad pH stability and good hydrolytic activity toward raffinose family oligosaccharides

文献类型： 外文期刊

作者： Hu, Yujing 1 ; Tian, Guoting 3 ; Zhao, Liyan 4 ; Wang, Hexiang 1 ; Tzi Bun Ng 5 ;

作者机构： 1.China Agr Univ, State Key Lab Agrobiotechnol, 2 Yuanmingyuan West St, Beijing 100193, Peoples R China

2.China Agr Univ, Dept Microbiol, 2 Yuanmingyuan West St, Beijing 100193, Peoples R China

3.Yunnan Acad Agr Sci, Inst Biotechnol & Germplasm Resource, Kunming 650223, Peoples R China

4.Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Jiangsu, Peoples R China

5.Chinese Univ Hong Kong, Fac Med, Sch Biomed Sci, Shatin, Hong Kong, Peoples R China

关键词： alpha-Galactosidase;Pleurotus djamor;Protease-resistant;Raffinose family oligosaccharides

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）

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收录情况： SCI

摘要： An acidic alpha-galactosidase designated as PDGI (Pleurotus djamor alpha-galactosidase) was purified to homogeneity with 290-fold purification and a specific activity of 52.18 units/mg by means of ion exchange chromatography and gel filtration chromatography. PDGI is a monomeric protein exhibiting a molecular mass of 60 kDa in SDS-PAGE and gel filtration. The optimum pH and temperature of the enzyme with pNPGal as substrate were 5.0 and 53.5 degrees C, respectively. It displayed great pH stability within the pH range 3.0-10.0. Besides, the enzyme harbored remarkable resistance to acid protease and varying degrees of tolerance to other proteases: trypsin > collagenase Type-I> alpha-chymotrypsin neutral protease > proteinase K. It was strongly inhibited by K-+,K- Cd2+, Cu2+, Hg2+, Al3+, Fe3+ and Ag+ ions. The chemical modification reagents diethypyrocarbonate (DEPC), 2,3-butanedione (DIC) and trinitrophenol (TNBS) increased the activity of PDGI 1.5-fold whereas N-bromosuccinimide (NBS) and parachloro-mercuri-benzoate (PCMB) drastically suppressed its activity. PDGI displayed activity toward stachyose and raffinose. The Km values for hydrolysis of pNPGal, stachyose and raffinose were 0.76, 7.63 and 6.29 mM, respectively. Furthermore, PDGI degraded raffinose and sthachyose. These results suggest that PDGI has great potential for elimination of the non-digestible and flatulence-causing oligosaccharides stachyose and raffinose from legumes. (C) 2016 Elsevier B.V. All rights reserved.

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