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Jasmonic acid and methyl dihydrojasmonate enhance saponin biosynthesis as well as expression of functional genes in adventitious roots of Panax notoginseng FH Chen

文献类型： 外文期刊

作者： Li, Jinxin 1 ; Wang, Juan 1 ; Wu, Xiaolei 2 ; Liu, Dahui 3 ; Li, Jing 1 ; Li, Jianli 4 ; Liu, Shujie 4 ; Gao, Wenyuan 1 ;

作者机构： 1.Tianjin Univ, Sch Pharmaceut Sci & Technol, Tianjin Key Lab Modern Drug Delivery & High Effic, Tianjin 300072, Peoples R China

2.Tianjin ZhongXin Pharmaceut R&D Ctr, Tianjin, Peoples R China

3.Yunnan Acad Agr Sci, Inst Med Plants, Kunming, Peoples R China

4.Tianjin Univ Sci & Technol, Key Lab Ind Fermentat Microbiol, Minist Educ, Tianjin, Peoples R China

关键词： antioxidant enzymes;gene;HPLC-ESI-MS/MS;jasmonic acid;methyl dihydrojasmonate

期刊名称：BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY （ 影响因子：2.431； 五年影响因子：2.124 ）

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收录情况： SCI

摘要： Panax notoginseng, an important herbal medicine, has wide uses for its bioactive compounds and health function. In this work, we compared the content of saponin in cultivation and adventitious root. The total content of saponins in adventitious root (8.48 mg.g(-1)) was found lower than in the native one (3-year-old) (34.34 mg.g(-1)). To enhance the content of bioactive compounds, we applied elicitors jasmonic acid (JA) and methyl dihydrojasmonate (MDJ) to the adventitious root culture. It was observed that the highest total content of saponins (71.94 mg.g(-1)) was achieved after treatment with 5 mg.L-1 JA, which was 2.09-fold higher than native roots and 8.45-fold higher than the control group. The findings from high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry analysis showed that six new compounds were present after the treatment with the elicitors. Furthermore, we found that JA and MDJ significantly upregulated the expression of the geranyl diphosphate synthase, farnesyl diphosphate synthase, squalene synthase, squalene epoxidase, dammarenediol synthase, and CYP716A47 and CYP716A53v2 (CYP450 enzyme) genes; downregulated the expression of the cycloartenol synthase gene; and increased superoxide dismutase and peroxidase activities. (C) 2016 International Union of Biochemistry and Molecular Biology, Inc.

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[1]Effect of Jasmonic Acid to Resistance against Fusarium in Lily. Zhang, Y. P.,Cui, G. F.,Wu, L. F.,Wang, J. H.,Tang, D. S.,Lee, I. J.. 2011

[2]Aspergillus niger Enhance Bioactive Compounds Biosynthesis As Well As Expression of Functional Genes in Adventitious Roots of Glycyrrhiza uralensis Fisch. Li, Jing,Wang, Juan,Li, Jinxin,Li, Hongfa,Gao, Wenyuan,Li, Jing,Liu, Dahui,Li, Jianli,Liu, Shujie.

[3]Analysis of ginsenoside content, functional genes involved in ginsenosides biosynthesis, and activities of antioxidant enzymes in Panax quinquefolium L. adventitious roots by fungal elicitors. Li, Jinxin,Li, Hongfa,Wang, Juan,Liu, Shujie,Li, Jianli,Liu, Dahui.

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