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Isolation of a protease-resistant and pH-stable alpha-galactosidase displaying hydrolytic efficacy toward raffinose family oligosaccharides from the button mushroom Agaricus bisporus

文献类型： 外文期刊

作者： Hu, Yujing 1 ; Zhu, Mengjuan 4 ; Tian, Guoting 5 ; Zhao, Liyan 6 ; Wang, Hexiang 2 ; Ng, Tzi Bun 7 ;

作者机构： 1.Beijing Univ Agr, Dept Plant Sci & Techonol, Beijing 102206, Peoples R China

2.China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China

3.China Agr Univ, Dept Microbiol, Beijing 100193, Peoples R China

4.Shandong Agr Univ, Dept Fungal Resource, 61 Daizong St, Tai An 271018, Shandong, Peoples R China

5.Yunnan Acad Agr Sci, Inst Biotechnol & Germplasm Resource, Kunming 650223, Yunnan, Peoples R China

6.Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Jiangsu, Peoples R China

7.Chinese Univ Hong Kong, Sch Biomed Sci, Fac Med, Shatin, Hong Kong, Peoples R China

关键词： alpha-galactosidase;Agaricus bisporus;Protease-resistant;Raffinose family oligosaccharides

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）

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收录情况： SCI

摘要： A 45-kDa monomeric acidic alpha-galactosidase with a specific activity of 193.12 units/mg was isolated from the fruiting bodies of Agaricus bisporus. Blast search of internal peptide sequences suggested that it is a member of GH family 27 and it is most similar to hypothetical protein AGABI2DRAFT_70106. The enzyme displayed maximal activity at pH 4.0 and 60 degrees C, respectively. The enzyme remained stable within the pH range 2.0-9.0 but its activity was markedly suppressed in the presence of Cu2+, Hg2+, Fe3+ and Ag+ ions. It displayed resistance to a-chymotrypsin and neutral protease. Moreover, it manifested degradative activity toward both oligosaccharides and polysaccharides. The enzyme manifested Km values of 0.30 mM, 10.65 mM and 19.21 mM, toward pNPGal, stachyose and raffinose respectively. These results suggest that Agaricus bisporus a-galactosidase is a promising candidate for elimination of raffinose oligosaccharides (RFOs) in biotechnological applications. (C) 2017 Elsevier B.V. All rights reserved.

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