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Clustering and cellular distribution characteristics of virus particles of Tomato spotted wilt virus and Tomato zonate spot virus in different plant hosts

文献类型： 外文期刊

作者： Zhang, Zhongkai 1 ; Zheng, Kuanyu 2 ; Dong, Jiahong 2 ; Fang, Qi 3 ; Hong, Jian; Wang, Xifeng 1 ;

作者机构： 1.Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, Inst Plant Protect, Beijing 100193, Peoples R China

2.Yunnan Acad Agr Sci, Biotechnol & Germplasm Resources Inst, Key Lab Southwestern Crop Gene Resources & Germpl, Minist Agr,Yunnan Prov Key Lab Agr Biotechnol, Kunming 650223, Yunnan, Peoples R China

3.Yunnan Acad Agr Sci, Biotechnol & Germplasm Resources Inst, Key Lab Southwestern Crop Gene Resources & Germpl, Minist Agr,Yunnan Prov Key Lab Agr Biotechnol, Kunming 650223, Yunnan, Peopl

关键词： Tomato spotted wilt virus;Tomato zonate spot virus;Virus particles;Clustering;Cellular distribution

期刊名称：VIROLOGY JOURNAL （ 影响因子：4.099； 五年影响因子：3.719 ）

ISSN： 1743-422X

年卷期： 2016 年 13 卷

页码：

收录情况： SCI

摘要： Background: Tomato spotted wilt virus (TSWV) and Tomato zonate spot virus (TZSV) are the two dominant species of thrip-transmitted tospoviruses, cause significant losses in crop yield in Yunnan and its neighboring provinces in China. TSWV and TZSV belong to different serogroup of tospoviruses but induce similar symptoms in the same host plant species, which makes diagnostic difficult. We used different electron microscopy preparing methods to investigate clustering and cellular distribution of TSWV and TZSV in the host plant species. Results: Negative staining of samples infected with TSWV and TZSV revealed that particles usually clustered in the vesicles, including single particle (SP), double particles clustering (DPC), triple particles clustering (TPC). In the immunogold labeling negative staining against proteins of TZSV, the antibodies against Gn protein were stained more strongly than the N protein. Ultrathin section and high pressure freeze (HPF)-electron microscopy preparations revealed that TSWV particles were distributed in the cisternae of endoplasmic reticulum (ER), filamentous inclusions (FI) and Golgi bodies in the mesophyll cells. The TSWV particles clustered as multiple particles clustering (MPC) and distributed in globular viroplasm or cisternae of ER in the top leaf cell. TZSV particles were distributed more abundantly in the swollen membrane of ER in the mesophyll cell than those in the phloem parenchyma cells and were not observed in the top leaf cell. However, TZSV virions were mainly present as single particle in the cytoplasm, with few clustering as MPC. Conclusion: In this study, we identified TSWV and TZSV particles had the distinct cellular distribution patterns in the cytoplasm from different tissues and host plants. This is the first report of specific clustering characteristics of tospoviruses particles as well as the cellular distribution of TSWV particles in the FI and globular viroplasm where as TZSV particles inside the membrane of ER. These results indicated that tospoviruses particles possessed specific and similar clustering in the saps of diseased plants. Furthermore, the results of this study will also provide a basis for further study on the tospoviruses assembling, maturation and movement.

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[1]Occurrence and diversity of Tomato spotted wilt virus isolates breaking the Tsw resistance gene of Capsicum chinense in Yunnan, southwest China. Jiang, L.,Huang, Y.,Sun, L.,Wang, B.,Zhu, M.,Li, J.,Tao, X.,Huang, C.,Liu, Y.,Li, F.,Liu, Y.,Dong, J.,Zhang, Z..

[2]Identification of three new isolates of Tomato spotted wilt virus from different hosts in China: molecular diversity, phylogenetic and recombination analyses. Shi, Kerong,Zhang, Zhenjia,Wang, Deya,Yu, Chengming,Wang, Zenghui,Yuan, Xuefeng,Dong, Jiahong. 2016

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