High-Throughput Development of SSR Markers from Pea (Pisum sativum L.) Based on Next Generation Sequencing of a Purified Chinese Commercial Variety
文献类型： 外文期刊
作者： Yang, Tao 1 ; Fang, Li 1 ; Zhang, Xiaoyan 2 ; Hu, Jinguo 3 ; Bao, Shiying 4 ; Hao, Junjie 2 ; Li, Ling; He, Yuhua 5 ; Jia 1 ;
作者机构： 1.Chinese Acad Agr Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Inst Crop Sci, Beijing 100193, Peoples R China
2.Qingdao Acad Agr Sci, Qingdao, Peoples R China
3.Washington State Univ, USDA ARS, Western Reg Plant Intro Stn, Pullman, WA 99164 USA
4.Yunnan Acad Agr Sci, Inst Grain Crops, Kunming, Peoples R China
5.Yunnan Acad Agr Sci, Inst Grain Crops, Kunming, Peop
期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）
ISSN： 1932-6203
年卷期： 2015 年 10 卷 10 期
页码：
收录情况： SCI
摘要： Pea (Pisum sativum L.) is an important food legume globally, and is the plant species that J.G. Mendel used to lay the foundation of modern genetics. However, genomics resources of pea are limited comparing to other crop species. Application of marker assisted selection (MAS) in pea breeding has lagged behind many other crops. Development of a large number of novel and reliable SSR (simple sequence repeat) or microsatellite markers will help both basic and applied genomics research of this crop. The Illumina HiSeq 2500 System was used to uncover 8,899 putative SSR containing sequences, and 3,275 non-redundant primers were designed to amplify these SSRs. Among the 1,644 SSRs that were randomly selected for primer validation, 841 yielded reliable amplifications of detectable polymorphisms among 24 genotypes of cultivated pea (Pisum sativum L.) and wild relatives (P. ful-vum Sm.) originated from diverse geographical locations. The dataset indicated that the allele number per locus ranged from 2 to 10, and that the polymorphism information content (PIC) ranged from 0.08 to 0.82 with an average of 0.38. These 1,644 novel SSR markers were also tested for polymorphism between genotypes G0003973 and G0005527. Finally, 33 polymorphic SSR markers were anchored on the genetic linkage map of G0003973 x G0005527 F-2 population.
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