A novel approach: enhancing marigold (Tagetes erecta L.) genetic transformation through seed priming technology
文献类型： 外文期刊
作者： Huang, Wang-Qi 1 ; Meng, Chao 1 ; Zhang, Lu 1 ; Xu, Feng 1 ; Yang, Xiu-Mei 1 ; Zhang, Li-Fang 1 ; Jiang, Ya-Lian 1 ; Shi, Rui-Xue 1 ; Zhao, A-Xiang 1 ; Zhang, Yi-Ping 1 ; Ali, Niaz 1 ; Chen, Xiu-Hua 3 ;
作者机构： 1.Yunnan Acad Agr Sci, Flower Res Inst, Natl Engn Res Ctr Ornamental Hort, Yunnan Flower Breeding Key Lab, Kunming, Peoples R China
2.Shaheed Benazir Bhutto Univ, Dept Agr, Sheringal, Dir Upper, Pakistan
3.Yunnan Acad Agr Sci, Int Agr Res Inst, Kunming, Peoples R China
关键词： pigmented marigold; tagetes erecta; genetic transformation; seed priming; vacuum infiltration
期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：4.8； 五年影响因子：5.7 ）
ISSN： 1664-462X
年卷期： 2024 年 15 卷
页码：
收录情况： SCI
摘要： This research presents an innovative genetic transformation protocol for marigolds (Tagetes erecta L.), a species of great significance in floriculture, impacting both yield and quality. The study introduces seed priming technology as a novel approach and evaluates its effect on the germination rate. The results indicate that the germination rates of pigmented marigold seeds were not significantly affected by Agrobacterium immersion under optimal conditions, although variations were observed in genotypes and treatment parameters. Optimal germination was observed at an optical density (OD600 nm) of 1.3 with a vacuum infiltration time of 10 min. The transgenic plants were confirmed through Basta herbicide resistance, Green florescent protein (GFP) fluorescence screening, and polymerase chain reaction (PCR) detection of the GFP gene. After the treatment, the morphological assessments showed genotype-dependent variations in plant height and fresh weight, while the biochemical analysis revealed significant variations in malondialdehyde (MDA) levels, peroxidase (POD), superoxide dismutase (SOD), and root activities. Additionally, the study examined the efficacy of various scarification techniques on seed survival rate, and seed coat removal was found to be the most effective method for marigold transformation. These findings provide a robust foundation for optimizing genetic transformation methods to enhance marigold crop resilience and quality within the floricultural sector.
- 相关文献
作者其他论文 更多>>
-
Herbicide Screening and Application Method Development for Sustainable Weed Management in Tagetes erecta L. Fields
作者：Zhang, Yiping;Huang, Wangqi;Xu, Feng;Jiang, Yalian;Wang, Jihua;Zhang, Yiping;Huang, Wangqi;Xu, Feng;Jiang, Yalian;Wang, Jihua;Zhang, Yiping;Huang, Wangqi;Xu, Feng;Jiang, Yalian;Wang, Jihua;Feng, Dongyan;Jia, Chengcheng;Tang, Dongsheng;Huang, Junhong;Li, Ye
关键词：weed management; pre-emergence herbicides; post-emergence herbicides; crop safety
-
Microscopic observation and transcriptome analysis provide insights into mechanisms of hybrid incompatibility in Rhododendron
作者：Xie, Weijia;Li, Shifeng;Peng, Lvchun;Wang, Jihua;Zhang, Lu;Song, Jie;Huang, Hui;Oba, Elias G.
关键词：Rhododendron; Interspecific hybridization; Intraspecific hybridization; Microscopic observation; Transcriptome analysis
-
Phosphorylated transcription factor PuHB40 mediates ROS-dependent anthocyanin biosynthesis in pear exposed to high light
作者：Zhang, Lu;Wang, Lu;Fang, Yongchen;Gao, Yuhao;Yang, Shulin;Ni, Junbei;Teng, Yuanwen;Bai, Songling;Su, Jun;Teng, Yuanwen
关键词：
-
EMS-Mediated Mutagenesis in Marigold Seeds and Its Effects on Seedling Growth and Physiology
作者：Meng, Chao;Zhang, Yiping;Shi, Ruixue;Jiang, Yalian;Huang, Wangqi;Ullah, Ikram;Wu, Wenjin;Luo, Shaodan;Luo, Cuixia;Nanda, Satyabrata;Seleiman, Mahmoud F.
关键词：Marigolds; seed germination; EMS mutagenesis; genetic enhancement
-
Chromosomal analysis of progenies between Lilium intersectional hybrids and wild species using ND-FISH and GISH
作者：Zhou, Mei;Yong, Xue;Zhu, Jungang;Xu, Qian;Zhang, Lu;Mou, Lisha;Zeng, Lijia;Wu, Mengxi;Jiang, Beibei;Jia, Yin;Liu, Xiaodan;Zhang, Peihua;Pan, Yuanzhi
关键词：
Lilium ; intersectional hybrids; wild species; cytogenetics; ND-FISH; GISH; oligonucleotide probes -
Genome and GWAS analysis identified genes significantly related to phenotypic state of Rhododendron bark
作者：Ye, Qiannan;Li, Qing;Zhou, Yanli;Wu, Zhenzhen;Hu, Yanting;Zhang, Chengjun;Ye, Qiannan;Li, Qing;Wu, Zhenzhen;Zhang, Lu;Wang, Jihua;Ji, Yaliang;Ma, Yongpeng;Zhang, Chengjun;Zhang, Chengjun
关键词：
-
Genome-Wide Identification of the RR Gene Family and Its Expression Analysis in Response to TDZ Induction in Rhododendron delavayi
作者：Peng, Lvchun;Zhao, Zhengxiong;Peng, Lvchun;Xie, Weijia;Zhang, Lu;Song, Jie;Li, Shifeng;Li, Xuejiao;Gao, Yan;Zhao, Zhengxiong
关键词：Rhododendron delavayi; adventitious bud regeneration; RR gene family; segmental replication
