Characterization of primary aroma compounds in Pu-erh raw tea sourced from various regions using gas chromatography-mass spectrometry and headspace solid-phase microextraction
文献类型： 外文期刊
作者： Du, Lijuan 1 ; Ye, Yanping 1 ; Shao, Jinliang 1 ; Wang, Yutong 1 ; Zhu, Guolei 3 ; Jiang, Hua 4 ; Liu, Hongcheng 1 ; Liu, Zhenhuan 1 ;
作者机构： 1.Yunnan Acad Agr Sci, Inst Qual Stand & Testing Tech, Kunming 650205, Peoples R China
2.Yunnan Agr Univ, Fac Food Sci & Technol, Kunming, Peoples R China
3.Southwest Forestry Univ, Key Lab State Forestry & Grassland Adm Highly Effi, Kunming, Peoples R China
4.Yunnan Huaying Technol Co Ltd, Kunming, Peoples R China
关键词： enantiomers; KEGG pathway; Pu-erh raw tea; volatiles
期刊名称：JOURNAL OF FOOD SCIENCE （ 影响因子：3.4； 五年影响因子：4.1 ）
ISSN： 0022-1147
年卷期： 2024 年 89 卷 12 期
页码：
收录情况： SCI
摘要： The assessment of aroma plays a crucial role in determining the characteristics and quality of Pu-erh tea. The volatile compounds present in Pu-erh raw tea sourced from five regions in Yunnan Province have been studied using gas chromatography-mass spectrometry (GC-MS) in conjunction with headspace solid phase microextraction (HS-SPME). A total of 69 aroma-active compounds were identified, with alcohols, ketones, and aldehydes being the predominant component types. Furthermore, notable variations were observed among four pairs of volatile terpenoid enantiomers. Significant discrimination was achieved using orthogonal partial least squares discrimination analysis (R(x(2)) = 0.926, R(y(2)) = 0.843, Q(2) = 0.501). Moreover, 33 important differential compounds were identified based on variable importance in the projection values (VIP > 1.0) and one-way ANOVA analysis (p < 0.05). Subsequently, a KEGG pathway analysis was conducted, revealing enrichment pathways primarily focused on the mevalonate pathway, steroid biosynthesis, and fatty acid biosynthesis. Ultimately, the key volatile compounds and major aroma-contributing compounds were successfully identified.
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