Molecular Diagnosis and Vegetative Compatibility Group Analysis of Fusarium Wilt of Banana in Nepal
文献类型： 外文期刊
作者： Pant, Bimala 1 ; Bai, Tingting 2 ; Du, Chanjuan 3 ; Baidya, Suraj 1 ; Magar, Prem Bahadur 1 ; Manandhar, Shrinkhala 1 ; Shrestha, Jiban 5 ; Dita, Miguel 6 ; Rouard, Mathieu 7 ; Fu, Gang 3 ; Zheng, Si-Jun 2 ;
作者机构： 1.Nepal Agr Res Council, Natl Plant Pathol Res Ctr, Lalitpur 44700, Bagmati Provinc, Nepal
2.Yunnan Acad Agr Sci, Agr Environm & Resources Inst, Yunnan Key Lab Green Prevent & Control Agr Transbo, Beijing Rd 2238, Kunming 650205, Peoples R China
3.Guangxi Acad Agr Sci, Plant Protect Res Inst, Guangxi Key Lab Biol Crop Dis & Insect Pests, Daxue Rd 174, Nanning 530007, Peoples R China
4.North Carolina State Univ, Dept Entomol & Plant Pathol, Raleigh, NC 27695 USA
5.Nepal Agr Res Council, Natl Plant Breeding & Genet Res Ctr, Lalitpur 44700, Bagmati Provinc, Nepal
6.Biovers Int, Palmira 763537, Colombia
7.Biovers Int, Parc Sci Agropolis II, F-34397 Montpellier 5, France
8.Biovers Int, Beijing Rd 2238, Kunming 650205, Peoples R China
关键词： Fusarium wilt of banana; Foc race 1; pathogen identification; Panama diseases; vegetative compatibility group
期刊名称：JOURNAL OF FUNGI （ 影响因子：4.7； 五年影响因子：5.2 ）
ISSN：
年卷期： 2023 年 9 卷 2 期
页码：
收录情况： SCI
摘要： Fusarium wilt of banana (FWB), caused by Fusarium oxysporum f. sp. cubense (Foc), is the most important constraint of the banana industry globally. In Nepal, epidemics resembling FWB have been increasingly observed on the Malbhog cultivar in the past several years. However, the disease has not been officially reported yet, and consequently, little is known about the pathogen present across the country. In this study, we characterized 13 fungal strains isolated from banana plants of the Malbhog cultivar (Silk, AAB) showing symptoms similar to FWB in banana plantations in Nepal. All of the strains were typed as belonging to the F. oxysporum and caused FWB symptoms when inoculated in the Malbhog and Cachaco (Bluggoe, ABB) cultivars. No symptoms were observed in the Williams cultivar (Cavendish, AAA). Vegetative compatibility group (VCG) analysis classified the strains as VCG 0124 or VCG 0125. PCR analyses conducted with primers specific for Foc race 1 (Foc R1) or Foc tropical race 4 (TR4) revealed that all the strains reacted positively for Foc R1 and none for TR4. Altogether, our results demonstrated that the pathogen populations causing FWB of the Malbhog cultivar in Nepal were Foc R1. This work reported, for the first time, the occurrence of FWB in Nepal. Further studies with larger Foc populations are needed to better understand disease epidemiology to design sustainable disease management strategies.
- 相关文献
作者其他论文 更多>>
-
Shaping the future of bananas: advancing genetic trait regulation and breeding in the postgenomics era
作者：Miao, Hongxia;Zhang, Jianbin;Zheng, Yunke;Jia, Caihong;Wang, Jingyi;Zhang, Jing;Sun, Peiguang;Jin, Zhiqiang;Zhou, Yongfeng;Wang, Wei;Xie, Jianghui;Liu, Juhua;Miao, Hongxia;Zhang, Jianbin;Jia, Caihong;Hu, Yulin;Wang, Jingyi;Zhang, Jing;Sun, Peiguang;Jin, Zhiqiang;Zhou, Yongfeng;Wang, Wei;Xie, Jianghui;Liu, Juhua;Miao, Hongxia;Zhang, Jianbin;Zheng, Yunke;Jia, Caihong;Wang, Jingyi;Zhang, Jing;Sun, Peiguang;Wang, Wei;Liu, Juhua;Hu, Yulin;Zhou, Yongfeng;Zheng, Sijun;Zheng, Sijun;Rouard, Mathieu
关键词：
-
Bacillus velezensis YN2111 reveals the potential role of amine oxidase in Fusarium wilt response in Cavendish bananas
作者：Yu, Hongwei;Zhou, Guangdong;Zhang, Wenlong;Liu, Kunhua;Fan, Huacai;Li, Shu;Zheng, Si-Jun;Yu, Hongwei;Zhou, Guangdong;Liu, Kunhua;Omondi, Bonaventure Aman;Cenci, Alberto;Rouard, Mathieu;Liu, Juhua;Zheng, Si-Jun
关键词：Bacillus velezensis; Biological control; Fusarium wilt of banana; Banana amine oxidase; Microbial-plant interactions
-
Effect of plant elicitor Isotianil on the rhizosphere soil microbiome composition of banana cultivars with different resistance to Fusarium wilt
作者：Zhang, Wenlong;Zhou, Guangdong;Yu, Hongwei;Tian, Libo;Zheng, Si-Jun;Zhang, Wenlong;He, Ping;Wang, Yun-Yue;Zheng, Si-Jun;Tian, Libo
关键词：Plant health; Plant elicitor; Rhizosphere; 16S and ITS; Co-occurrence network; Bacterial community assembly
-
Isolation and Characterization of Beauveria caledonica (Ascomycota: Hypocreales) Strains for Biological Control of Odoiporus longicollis Oliver (Coleoptera: Curculionidae)
作者：Ding, Mingbi;Wu, Li;Yu, Hongwei;Fan, Huacai;Guo, Zhixiang;Xu, Shengtao;Wang, Yongfen;Zheng, Si-Jun;Ding, Mingbi;Wu, Li;Yu, Hongwei;Chun, Jianhui;Wang, Yongfen;Zheng, Si-Jun
关键词：
O. longicollis (Oliver);B. caledonica ; biological characteristics; virulence; greenhouse evaluation -
A smooth vetch (Vicia villosa var.) strain endogenous to the broad-spectrum antagonist Bacillus siamensis JSZ06 alleviates banana wilt disease
作者：Ruan, Yan-Nan;Nong, Caihong;Fan, Huacai;Fu, Libo;Zheng, Si-Jun;Li, Shu;Wang, Zhi-Yuan;Ruan, Yan-Nan;Nong, Caihong;Jintrawet, Attachai
关键词：smooth vetch; Bacillus siamensis; Fusarium wilt of banana; mechanism of inhibition; pot experiment; LC-MS/MS; biological control
-
The interaction between Fusarium oxysporum f. sp. cubense tropical race 4 and soil properties in banana plantations in Southwest China
作者：Zhang, Wenlong;Bai, Tingting;Jamil, Arslan;Fan, Huacai;Li, Xundong;Zheng, Si-Jun;Xu, Shengtao;Zheng, Si-Jun
关键词：Banana Fusarium wilt; Physiochemical properties; Soil microbial communities; Pathogenic fungal abundance
-
The Dual Effect of Selenium Application in Reducing Fusarium Wilt Disease Incidence in Banana and Producing Se-Enriched Fruits
作者：Liu, Lina;Yin, Kesuo;Ni, Ming;Ding, Yue;Zheng, Si-Jun;Liu, Lina;Li, Chengyun;Wang, Chengye;Zheng, Si-Jun
关键词：banana; Fusarium oxysporum f. sp. cubense tropical race 4; Se-enriched fruits; selenium; antioxidant enzymes
