Molecular characterization and pathogenicity of an infectious cDNA clone of tomato brown rugose fruit virus
文献类型： 外文期刊
作者： Ma, Ziyue 1 ; Zhang, Hui 2 ; Ding, Ming 3 ; Zhang, Zhongkai 3 ; Yang, Xiuling 1 ; Zhou, Xueping 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China
2.Shanghai Acad Agr Sci, Hort Res Inst, Shanghai 201106, Peoples R China
3.Yunnan Acad Agr Sci, Inst Biotechnol & Germplasm Resources, Yunnan Prov Key Lab Agr Biotechnol, Kunming 650223, Yunnan, Peoples R China
4.Zhejiang Univ, Inst Biotechnol, State Key Lab Rice Biol, Hangzhou 310058, Peoples R China
关键词： Tobamovirus; Tomato brown rugose fruit virus; Infectious clone; Agroinoculation
期刊名称：PHYTOPATHOLOGY RESEARCH （ 影响因子：3.955； 五年影响因子：3.955 ）
ISSN： 2096-5362
年卷期： 2021 年 3 卷 1 期
页码：
收录情况： SCI
摘要： Tomato brown rugose fruit virus (ToBRFV) is a new member of the genus Tobamovirus, and has the potential to affect the production and marketability of tomatoes and peppers. In this study, we sequenced and analyzed the complete genome of ToBRFV isolates from tomato plants showing mosaic and mottling symptoms in Yunnan Province of China. We constructed a full-length infectious cDNA clone of ToBRFV, which could induce systemic infection with typical symptoms in tomato, Nicotiana benthamiana, and N. tabacum cv. Samsun nn plants through Agrobacterium-mediated inoculation. Further experimental evidence demonstrated that the rod-shaped virions accumulating in agroinfiltrated plants are sap-transmissible. This is the first report on the construction of a biologically active, full-length infectious cDNA clone of ToBRFV. The system developed herein will facilitate further research on functions of ToBRFV-encoded proteins and plant-ToBRFV interactions through reverse genetic approaches.
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