文献类型： 外文期刊
作者： Liu, Hai-Jun 1 ; Jian, Liumei 1 ; Xu, Jieting 1 ; Zhang, Qinghua 1 ; Zhang, Maolin 1 ; Jin, Minliang 1 ; Peng, Yong 1 ; Y 1 ;
作者机构： 1.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China
2.WIMI Biotechnol Co Ltd, Changzhou 213000, Peoples R China
3.Yunnan Acad Agr Sci, Xishuangbanna Inst Agr Sci, Kunming 650205, Yunnan, Peoples R China
4.Jilin Acad Agr Sci, Agrobiotechnol Inst, Jilin Prov Key Lab Agr Biotechnol, Changchun 130033, Peoples R China
5.Biogle Genome Editing Ctr, Changzhou 213125, Peoples R China
6.Beijing Acad Agr & Forestry Sci, Beijing Key Lab Maize DNA Fingerprinting & Mol Br, Beijing 100097, Peoples R China
7.Cold Spring Harbor Lab, POB 100, Cold Spring Harbor, NY 11724 USA
8.Max Planck Inst Mol Plant Physiol, D-14476 Potsdam, Germany
期刊名称：PLANT CELL （ 影响因子：11.277； 五年影响因子：12.061 ）
ISSN： 1040-4651
年卷期： 2020 年 32 卷 5 期
页码：
收录情况： SCI
摘要： Maize (Zea mays) is one of the most important crops in the world. However, few agronomically important maize genes have been cloned and used for trait improvement, due to its complex genome and genetic architecture. Here, we integrated multiplexed CRISPR/Cas9-based high-throughput targeted mutagenesis with genetic mapping and genomic approaches to successfully target 743 candidate genes corresponding to traits relevant for agronomy and nutrition. After low-cost barcode-based deep sequencing, 412 edited sequences covering 118 genes were precisely identified from individuals showing clear phenotypic changes. The profiles of the associated gene-editing events were similar to those identified in human cell lines and consequently are predictable using an existing algorithm originally designed for human studies. We observed unexpected but frequent homology-directed repair through endogenous templates that was likely caused by spatial contact between distinct chromosomes. Based on the characterization and interpretation of gene function from several examples, we demonstrate that the integration of forward and reverse genetics via a targeted mutagenesis library promises rapid validation of important agronomic genes for crops with complex genomes. Beyond specific findings, this study also guides further optimization of high-throughput CRISPR experiments in plants.
- 相关文献
作者其他论文 更多>>
-
Population dynamics and molecular adaption of Tetranychus cinnabarinus to long-term thermal stress
作者：Chen, Li;Ma, Ting;Liu, Jie;He, Lin;Shen, GuangMao;Shen, GuangMao
关键词：high temperature; Tetranychus cinnabarinus; thermal adaption; life table; heat shock protein
-
ZmWAK02 encoding an RD-WAK protein confers maize resistance against gray leaf spot
作者：Dai, Zhikang;Pi, Qianyu;Liu, Yutong;Hu, Long;Li, Bingchen;Zhang, Bao;Li, Wenqiang;Gui, Songtao;Lai, Zhibing;Pi, Qianyu;Liu, Yutong;Zhang, Bao;Lai, Zhibing;Pi, Qianyu;Liu, Yutong;Zhang, Bao;Lai, Zhibing;Wang, Yanbo;Jiang, Min;Qi, Xin;Llaca, Victor;Fengler, Kevin;Thatcher, Shawn;Li, Ziwei;Liu, Xiangguo;Fan, Xingming;Lai, Zhibing
关键词：gray leaf spot; maize; resistance; ZmWAK02
-
Repeated intercontinental migrations and recurring hybridizations characterise the evolutionary history of yew (Taxus L.)
作者：Moller, Michael;Liu, Jie;Ye, Lin-Jiang;Li, De-Zhu;Gao, Lian-Ming;Liu, Jie;Ye, Lin-Jiang;Li, De-Zhu;Moller, Michael;Mill, Robert;Thomas, Philip;Li, Yan;Li, Jian-Hua;Ye, Lin-Jiang;Li, De-Zhu
关键词：Ancestral area estimation; Biogeographic origin; Evolutionary history; Hybridization; Phylogenetic relationships; Vicariance
-
Analysis of crotonaldehyde puff-by-puff release in mainstream cigarette smoke under various smoking regimens by high-performance liquid chromatography with the modified QuEChERS method
作者：Li, Chao;Zhang, Jian;Tao, Ying;Zhang, Chengming;Shen, Xiaofeng;Liu, Ya;Qin, Yunhua;Li, E'Xian;Li, Chao;Li, E'Xian
关键词：RM20H smoking machine; QuEChERS; intensive smoking regimens; crotonaldehyde; puff-by-puff release
-
Domestication Origin and Breeding History of the Tea Plant (&ITCamellia&IT &ITsinensis&IT) in China and India Based on Nuclear Microsatellites and cpDNA Sequence Data
作者：Meegahakumbura, Muditha K.;Wambulwa, Moses C.;Li, Miao-Miao;Liu, Jie;Li, De-Zhu;Gao, Lian-Ming;Meegahakumbura, Muditha K.;Wambulwa, Moses C.;Yang, Jun-Bo;Li, De-Zhu;Meegahakumbura, Muditha K.;Wambulwa, Moses C.;Li, Miao-Miao;Li, De-Zhu;Meegahakumbura, Muditha K.;Wambulwa, Moses C.;Thapa, Kishore K.;Sun, Yong-Shuai;Moller, Michael;Xu, Jian-Chu;Liu, Ben-Ying
关键词：Camellia sinensis;nSSR;cpDNA sequence;domestication center;breeding history
-
How Red Mud-Induced Enhancement of Iron Plaque Formation Reduces Cadmium Accumulation in Rice with Different Radial Oxygen Loss
作者：Liu, Zhiyan;Yang, Junxing;Wan, Xiaoming;Peng, Yisheng;Liu, Jie;Wang, Xuedong;Zeng, Min
关键词：cadmium pollution;paddy soil;remediation;red mud;rice
-
Toxic effects of virtako on the brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae)
作者：Chen, Yong;Qing, Xiaowa;Liu, Jie;Zhang, Runjie;Chen, Yong;Qing, Xiaowa;Liu, Jie;Zhang, Runjie;Zhang, Jie
关键词：Brown planthopper;Toxicity;Laboratory assays;Lethal concentrations
