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Transcription factor PyHY5 binds to the promoters of PyWD40 and PyMYB10 and regulates its expression in red pear 'Yunhongli No. 1'

文献类型： 外文期刊

作者： Wang, Yuying 1 ; Zhang, Xiaodong 3 ; Zhao, Yiran 1 ; Yang, Jin 4 ; He, Yingyun 2 ; Li, Guochang 5 ; Ma, Weirong 6 ; Huang 1 ;

作者机构： 1.Yunnan Agr Univ, Coll Hort & Landscape, Kunming 650201, Yunnan, Peoples R China

2.Yunnan Acad Agr Sci, Inst Hort, Kunming 650205, Yunnan, Peoples R China

3.Coll Food & Bioengn Xuchang, Xuchang 461000, Henan, Peoples R China

4.Yunnan Acad Agr Sci, Ind Crop Inst, Kunming 650205, Yunnan, Peoples R China

5.Stn Shi Lin Ind Crop, Shilin 652200, Yunnan, Peoples R China

6.Stn Hong He Ind Crop, Mengzi 654400, Yunnan, Peoples R China

关键词： Red pear; HY5; MYB10; WD40; Anthocyanin accumulation

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：4.27； 五年影响因子：4.816 ）

ISSN： 0981-9428

年卷期： 2020 年 154 卷

页码：

收录情况： SCI

摘要： 'Yunhongli No. 1' is a rare and well-colored red pear (Pyrus pyrifolia) germplasm resource, and is popular in the market due to its bright red color and high quality. Light induces the expression of transportation factor genes MYB10, WD40, and HY5, which then activate the expression of critical genes in the anthocyanin biosynthesis pathway to promote the synthesis and accumulation of anthocyanin, thus giving the red coloration. Protein HY5 is considered to be a key regulator for induction of anthocyanin biosynthesis. The MYB10 genes physically interact with HY5 to positively regulate anthocyanin biosynthesis in Arabidopsis, apple, and pear by binding to G-box motifs. However, how these transcription factors are regulated by sunlight remains unclear in 'Yunhongli No. 1'. In this study, the transcription factor PyHY5 was cloned, and subcellular localization assay showed that PyHY5 was distributed in the nucleus. The DNA fragments of PyHY5 had a typical BRLZ domain of the bZIP family, and then were aligned against the promoter sequences of PyMYB10 and PyVVD40. Electrophoretic mobility shift and transient expression assays showed that PyHY5 could directly recognize and bind to the G-box motifs in the promoters of PyMYB10 and PyVVD40, and so boosted transcriptional activation by co-expression. The results demonstrated that PyHY5 binding to G-box motifs of the promoters of PyMYB10 and PyVVD40, enhanced its expression, and then promoted accumulation of anthocyanin in red 'Yunhongli No. 1'.

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