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Characterization of a New Orthotospovirus from Chilli Pepper in Yunnan Province, China

文献类型： 外文期刊

作者： Zheng, Kuanyu 1 ; Chen, Tsung-Chi 4 ; Wu, Kuo 1 ; Kang, Ya-Chi 4 ; Yeh, Shyi-Dong 6 ; Zhang, Zhongkai 1 ; Dong, Jiahon 1 ;

作者机构： 1.Yunnan Acad Agr Sci, Biotechnol & Germplasm Resources Inst, 2238 Beijing Rd, Kunming 650205, Yunnan, Peoples R China

2.Yunnan Prov Key Lab Agr Biotechnol, 2238 Beijing Rd, Kunming 650205, Yunnan, Peoples R China

3.Minist Agr, Key Lab Southwestern Crop Gene Resources & Germpl, 2238 Beijing Rd, Kunming 650205, Yunnan, Peoples R China

4.Asia Univ, Dept Biotechnol, Taichung 41354, Taiwan

5.China Med Univ, China Med Univ Hosp, Dept Med Res, Taichung 40402, Taiwan

6.Natl Chung Hsing Univ, Dept Plant Pathol, Taichung 402, Taiwan

7.Yunnan Univ Chinese Med, Coll Pharmaceut Sci, Kunming 650500, Yunnan, Peoples R China

关键词： genome; orthotospovirus; phylogeny

期刊名称：PLANT DISEASE （ 影响因子：4.438； 五年影响因子：4.7 ）

ISSN： 0191-2917

年卷期： 2020 年 104 卷 4 期

页码：

收录情况： SCI

摘要： Chilli pepper (Capsicum annuum L.) is one of the most important crops in Yunnan Province, China. An orthotospovirus isolate 14YV855 was isolated from a diseased chilli pepper plant exhibiting yellow ringspots and necrosis on leaves in Shiping County, Honghe Hani and Yi Autonomous Prefecture, Yunnan Province in 2014. The complete genome sequence of 14YV855 was determined. The small, medium, and large RNAs are 3,428, 4,781, and 8,917 nucleotides long, respectively. The complete nucleocapsid (N) protein of 14YV855 shares a high amino acid identity of 84.8 to 89.9% to that of Capsicum chlorosis virus (CaCV), Groundnut bud necrosis virus (GBNV), Watermelon bud necrosis virus (WBNV), and Watermelon silver mottle virus (WSMoV), which is slightly less than the 90% identity threshold for the demarcation of new Orthotospovirus sp. Phylogenetic analyses revealed that the N protein and RNA-dependent RNA polymerase of 14YV855 are the most related to WSMoV, while the NSs, NSm, and Gn/Gc proteins are similar to those of GBNV. As expected, 14YV855 is serologically related to CaCV, GBNV, WBNV, and WSMoV when the monoclonal antibody against the N protein of WSMoV was used; however, 14YV855 can be distinguished from other orthotospoviruses by reverse-transcription PCR using the specific primers. Our results indicate that 14YV855 is a new Orthotospovirus sp. belonging to the WSMoV serogroup and is provisionally named Chilli yellow ringspot virus.

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