Creation of Bacterial Blight Resistant Rice by Targeting Homologous Sequences of Xa13 and Xa25 Genes
文献类型： 外文期刊
作者： Zhu, Yiwang 1 ; Yang, Xiaohuai 3 ; Luo, Peirun 3 ; Yan, Jingwan 1 ; Cao, Xinglan 2 ; Qian, Hongge 2 ; Zhu, Xiying 2 ; Fan, Yujin 2 ; Mei, Fating 1 ; Fan, Meiying 1 ; Shang, Lianguang 2 ; Wang, Feng 1 ; Zhang, Yu 5 ;
作者机构： 1.Fujian Acad Agr Sci, Inst Biotechnol, Fujian Prov Key Lab Genet Engn Agr, Fuzhou 350003, Peoples R China
2.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen Branch, Genome Anal Lab Minist Agr & Rural Affairs,Guangdo, Shenzhen 518124, Peoples R China
3.Shenzhen Agr Sci & Technol Promot Ctr, Shenzhen 518000, Peoples R China
4.Henan Univ, Sch Life Sci, State Key Lab Crop Stress Adaptat & Improvement, Kaifeng 475004, Peoples R China
5.Food Crops Res Inst, Yunnan Acad Agr Sci YAAS, Yunnan Seed Lab, Yunnan Key Lab Rice Genet Improvement, Kunming 650200, Peoples R China
关键词： bacterial blight resistant; CRISPR/Cas9; Xa13; Xa25; rice
期刊名称：AGRONOMY-BASEL （ 影响因子：3.7； 五年影响因子：4.0 ）
ISSN：
年卷期： 2024 年 14 卷 4 期
页码：
收录情况： SCI
摘要： Bacterial blight is a destructive disease in rice caused by Xanthomonas oryzae pv. oryzae (Xoo). Single resistance genes often have limitations in providing broad-spectrum resistance, as pathogens continuously evolve and vary. Breeding rice varieties with multiple disease resistance genes has proven to be an effective strategy for controlling bacterial blight. In this study, a single Cas9/gRNA construct was used to target the homologous sequences of Xa13 and Xa25 genes through destroying the target gene function, creating bacterial blight resistance in five rice varieties. These materials provide promising germplasm resources for the development of rice varieties with durable resistance to bacterial blight.
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