Identification and Validation of Reference Genes for qRT-PCR Analysis of Petal-Color-Related Genes in Rosa praelucens
文献类型： 外文期刊
作者： Jian, Hongying 1 ; Wang, Huichun 1 ; Qiu, Xianqin 1 ; Yan, Huijun 1 ; Ma, Lulin 1 ;
作者机构： 1.Yunnan Acad Agr Sci, Flower Res Inst, Kunming 650205, Peoples R China
关键词： Rosa praelucens Byhouwer; petal color variation; qRT-PCR; reference gene; petal-color-related genes
期刊名称：GENES （ 影响因子：3.5； 五年影响因子：3.9 ）
ISSN：
年卷期： 2024 年 15 卷 3 期
页码：
收录情况： SCI
摘要： The flower's color is regarded as one of the most outstanding features of the rose. Rosa praelucens Byhouwer, an endemic and critically endangered decaploid wild rose species, is abundant in phenotypic diversity, especially in flower color variation, from white to different degrees of pink. The mechanism underlying this variation, e.g., the level of petal-color-related genes, is worth probing. Seven candidate reference genes for qRT-PCR analysis, including tubulin alpha chain (TUBA), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), histone H2B (Histone2A), eukaryotic translation elongation factor 1-alpha (EEF1A), 60S ribosomal protein (RPL37), eukaryotic translation initiation factor 1-alpha (EIF1A), and aquaporins (AQP), were detected from the transcriptome datasets of full blooming flowers of white-petaled and pink-petaled individuals, and their expression stabilities were evaluated through qRT-PCR analysis. According to stability rankings analysis, EEF1A showed the highest stability and could be chosen as the most suitable reference gene. Moreover, the reliability of EEF1A was demonstrated via qRT-PCR analysis of six petal-color-related target genes, the expression patterns of which, through EEF1A normalization, were found to be consistent with the findings of transcriptome analysis. The result provides an optimal reference gene for exploring the expression level of petal-color-related genes in R. praelucens, which will accelerate the dissection of petal-color-variation mechanisms in R. praelucens.
- 相关文献
作者其他论文 更多>>
-
Intraspecific rDNA FISH-based karyological variation corresponding to the phenotypic diversity of Rosa praelucens Byhouwer
作者：Jian, Hongying;Fang, Qiao;Cao, Shirui;Zhang, Ting;Tian, Min;Tang, Kaixue;Fang, Qiao;Cao, Shirui
关键词：Aneu-nonaploid; Fluorescence in situ hybridization; Karyological variation; Nonaploid; Phenotypic diversity; Rosa praelucens
-
Evolution of the biosynthetic pathways of terpene scent compounds in roses
作者：Shang, Junzhong;Liu, Heng;Niu, Lintao;Li, Runhui;Li, Yajun;Chen, Mengxi;Li, Ao;He, Yanhong;Bao, Manzhu;Ning, Guogui;Shang, Junzhong;Liu, Heng;Niu, Lintao;Li, Runhui;Li, Yajun;Chen, Mengxi;Li, Ao;He, Yanhong;Bao, Manzhu;Ning, Guogui;Feng, Dedang;Jian, Hongying;Tang, Kaixue;Wang, Jihua;Yan, Huijun;Liu, Zhenhua;Gao, Xiang;Wang, Changquan;Wang, Changquan;Yang, Shuhua
关键词：
-
Screening and Validation of Internal Reference Genes for Quantitative Real-Time PCR Analysis of Leaf Color Mutants in Dendrobium officinale
作者：Cao, Hua;Li, Han;Lu, Lin;Ma, Lulin;Ji, Yulu;Li, Shenchong
关键词：Dendrobium officinale; leaf color; qRT-PCR; reference gene; leaf color mutant
-
Ethylene controls cambium stem cell activity via promoting local auxin biosynthesis
作者：Yu, Qin;Cheng, Chenxia;Zhou, Xiaofeng;Yang, Chun;Zhou, Yingying;Gao, Junping;Ma, Nan;Cheng, Chenxia;Li, Yonghong;Hu, Yingchun;Soliman, Tarek M. A.;Zhang, Hao;Wang, Qigang;Wang, Huichun;Jiang, Cai-Zhong;Jiang, Cai-Zhong;Gan, Su-Sheng
关键词：auxin; cambium; ethylene; HD-ZIP I transcription factor; Rosa hybrida
-
Heterologous Expression of Platycodon grandiflorus PgF3′5′H Modifies Flower Color Pigmentation in Tobacco
作者：Ma, Lulin;Jia, Wenjie;Duan, Qing;Du, Wenwen;Li, Xiang;Cui, Guangfen;Wang, Xiangning;Wang, Jihua
关键词：PgF3'5'H; transgenic tobacco; over-expression; delphinidin-based anthocyanin; blue flower color
-
Ectopic Expression of PgF3 ' 5 ' H in Commercial Gypsophila paniculata Cultivar through Optimized Agrobacterium-Mediated Transformation
作者：Jin, Chunlian;Sun, Dan;Ma, Lulin;Mo, Xijun;Yang, Chunmei;Li, Fan
关键词：Gypsophila paniculata; regeneration; genetic transformation; commercial cultivar; PgF3 ' 5 ' H
-
Comprehensive Identification of Reliable Reference Genes for qRT-PCR Normalization of Fusarium oxysporum-Resistant Genes' Expressions in Lilium sargentiae Wilson
作者：Ma, Lulin;Li, Xiang;Duan, Qing;Jia, Wenjie;Du, Wenwen;Wang, Xiangning;Cui, Guangfen;Zhang, Yiping;Wang, Jihua
关键词：Lilium sargentiae Wilson; F; oxysporum inoculation; gene expression; stability
