Effect of cultivation years on saponins in Paris Polyphylla var. yunnanensis using ultra-high liquid chromatography-tandem mass spectrometry and Fourier transform infrared spectroscopy
文献类型： 外文期刊
作者： Wang, Yuan-Zhong 1 ; Li, Ping 1 ;
作者机构： 1.China Pharmaceut Univ, State Key Lab Nat Med, 24 Tongjia Lane, Nanjing 210009, Jiangsu, Peoples R China
2.Yunnan Acad Agr Sci, Inst Med Plants, 2238 Beijing Rd, Kunming 650200, Yunnan, Peoples R China
关键词： Paris Polyphylla var. yunnanensis;Cultivation year;Steroid saponins;UHPLC-MS/MS;FT-IR
期刊名称：PLANT GROWTH REGULATION （ 影响因子：3.412； 五年影响因子：3.691 ）
ISSN： 0167-6903
年卷期： 2018 年 84 卷 2 期
页码：
收录情况： SCI
摘要： Paris Polyphylla var. yunnanensis (PPY) has been used in traditional medicine for the treatment of parotitis and abscess. Previous studies showed the quality of PPY was associated with cultivation years. In this study, we applied ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) and Fourier transform infrared (FT-IR) spectroscopy to measure the variation of saponins due to cultivation years in cultivated PPY. A rapid UHPLC-MS/MS method was developed for simultaneous determination of six major steroidal saponins (polyphyllin I, II, V, VI, VII and gracillin). Principal component analysis (PCA) was selected for pattern recognition of FT-IR profiles. The concentration of six targeted steroid saponins varied with the increase of cultivation year and attained the maximum level at 8th year (22.65 +/- 1.65 mg g(-1)). A dynamic change with the growth of PPY presented in the content of diosgenin-type saponins and pennogenin-type saponins. After the treatment of standard normal variate (SNV), the PCA result showed that 4th and 5th years' samples were solely classified into two parts and the chemical information of other samples was quite similar. According to FT-IR and UPLC-MS/MS results, an optimal harvest time recommend at 7th or 8th year in the current stage. These results could support the previous view that the variations of metabolites were associated with cultivation years and provided chemical evidence for further research on the agricultural practice of PPY.
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