Qualitative and quantitative analysis of chemical constituents in Ardisiae Japonicae Herba
文献类型： 外文期刊
作者： Yu, Ke-Yun 1 ; Gao, Wen 2 ; Li, Shang-Zhen 1 ; Wu, Wei 3 ; Li, Pei 1 ; Dou, Li-Li 1 ; Wang, Yuan-Zhong 4 ; Liu, E-Hu 1 ;
作者机构： 1.China Pharmaceut Univ, State Key Lab Nat Med, 24 Tongjia Lane, Nanjing 210009, Jiangsu, Peoples R China
2.Shineway Pharmaceut Grp Co Ltd, Shijiazhuang, Hebei, Peoples R China
3.Guilin Med Univ, Coll Pharm, Guilin, Peoples R China
4.Yunnan Acad Agr Sci, Inst Med Plants, Kunming, Yunnan, Peoples R China
关键词： Ardisiae Japonicae Herba;chemical constituents;quality control;traditional Chinese medicine
期刊名称：JOURNAL OF SEPARATION SCIENCE （ 影响因子：3.645； 五年影响因子：2.943 ）
ISSN： 1615-9306
年卷期： 2017 年 40 卷 22 期
页码：
收录情况： SCI
摘要： Ardisiae Japonicae Herba is a well-known traditional Chinese medicine for the treatment of bronchitis conjunctivitis, pneumonia, and trauma. In this work, a high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry method was first established for the separation and structural identification of the chemical constituents in Ardisiae Japonicae Herba. A total of 15 compounds including coumarins, flavonoid glycosides, and catechins were identified or tentatively characterized based on their chromatographic behaviors and mass spectral fragmentation and by comparisons with the reference standards. Furthermore, a simple high-performance liquid chromatography with diode array detection method was developed for the simultaneous determination of five major constituents. Results obtained from method validation, including linearity, precision, repeatability, stability, and recovery, showed that the established method was reliable and accurate. Bergenin and quercitrin were found to be the most abundant constituents and could be served as chemical markers for quality control of Ardisiae Japonicae Herba.
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