Genome-Wide Identification of the GRAS Transcription Factor Family in Sweet Orange and the Regulation of Salt Stress-Enhanced Plant Salt Tolerance in Sweet Orange by CsGRAS15 and CsGRAS27
文献类型： 外文期刊
作者： Ren, Hailin 1 ; Xu, Rong 2 ; Wang, Jie 1 ; Zhang, Qian 1 ; Nie, Lili 1 ; Zhang, Li 3 ; Zhou, Xianyan 4 ; Liu, Xiaozhen 1 ; Zhang, Hanyao 1 ;
作者机构： 1.Southwest Forestry Univ, Forestry Coll, Kunming 650224, Peoples R China
2.Yuxi Normal Univ, Sch Chem Biol & Environm, Yuxi 653100, Peoples R China
3.Yunnan Agr Technol Extens Stn, Kunming 650000, Peoples R China
4.Yunnan Acad Agr Sci, Inst Trop & Subtrop Econ Crops, Baoshan 678000, Peoples R China
关键词： sweet orange; salt-responsive; GRAS transcription factor; signaling pathway
期刊名称：BIOMOLECULES （ 影响因子：4.8； 五年影响因子：5.6 ）
ISSN：
年卷期： 2025 年 15 卷 7 期
页码：
收录情况： SCI
摘要： Background: GRAS transcription factors are crucial for plant development and stress responses but remain poorly characterized in citrus. Soil salinization increasingly threatens sweet orange (Citrus sinensis) yield. Identifying salt-responsive GRAS genes could reveal key tolerance determinants for breeding resistant cultivars. Methods: We systematically identified and analyzed sweet orange GRAS transcription factors using bioinformatics. Results: Forty-three CsGRAS genes were identified, phylogenetically classified into ten subfamilies, and found to be structurally conserved. A promoter analysis revealed a high prevalence (58.78%) of hormone- and stress-responsive cis-elements. These genes reside on nine chromosomes, with segmental duplication being the primary evolutionary driver (eight duplicated pairs). Functional enrichment implicated hormone signaling pathways in regulating growth under stress. Transcriptome profiling identified 42 differentially expressed CsGRAS genes (19 upregulated and 23 downregulated) under salt stress. qRT-PCR validated the expression patterns of selected genes (e.g., CsGRAS15 and CsGRAS27). Notably, DELLA subfamily members CsGRAS15 and CsGRAS27, key negative regulators in gibberellin (GA) signaling, were differentially expressed. Modulating these DELLA proteins presents a promising strategy to enhance sweet orange salt tolerance by mitigating GA-mediated growth inhibition during stress. Conclusion: This study identifies salt-responsive CsGRAS genes, highlighting CsGRAS15 and CsGRAS27 as potential targets for improving salt tolerance in citrus.
- 相关文献
作者其他论文 更多>>
-
Integrated transcriptome and metabolome analyses reveal the mechanism by which bagging treatment affects peel reddening in Orah mandarin
作者：Wen, Ke;Li, Xulin;Yin, Tuo;Liu, Xiaozhen;Chen, Chaoying;Zi, Yinqiang;Zhao, Ke;Zhang, Hanyao;Pu, Jinan;Yan, Wenxiu;Wang, Xuemei;Zhou, Xianyan
关键词：Orah mandarin; Peel reddening; Bagging treatment; Transcriptome; Metabolome
-
Identification of the bHLH Transcription Factor Family in Orah Mandarin and the Response of CrbHLH46 to Low-Temperature Stress
作者：Chen, Chaoying;Yin, Tuo;Tian, Ping;Zhao, Ke;Zhang, Hanyao;Li, Xulin;Wen, Ke;Liu, Xiaozhen;Zhang, Li;Zhou, Xianyan
关键词：Orah mandarin; bHLH transcription factor; transcriptome; low-temperature stress; expression pattern
-
Comparative Global Metabolome Profile and Transcriptome Sequence Analysis of the Rough and Smooth Peel of the Orah Mandarin (Citrus reticulata)
作者：Liu, Hongming;Long, Chunrui;Fu, Xiaomeng;Wang, Shaohua;Lou, Yuqiang;Dong, Jianmei;Dong, Meichao;Mao, Jiamei;Yang, Jiandong;Yang, Hongxia;Du, Yuxia;Zhou, Xianyan
关键词：comparative omics; citrus fruit aesthetics; fruit quality; mandarin; phytohormone signaling; rough peels
-
Genome-wide identification of the MYB transcription factor family in kiwifruit and analysis of its expression pattern in response to salt stress
作者：Chen, Chaoying;Zhao, Wenjuan;Zi, Yinqiang;Zhao, Ke;Zhang, Hanyao;Li, Xulin;Wen, Ke;Liu, Xiaozhen;Chen, Daming
关键词：Kiwifruit; MYB transcription factor; Salt stress response; Expression analysis; Signaling pathway
-
Metabolome and transcriptome analyses reveal that pollination with 'Guanxi' honey pomelo pollen alleviates the postharvest fruit granulation of 'crystal' honey pomelo
作者：Yang, Jing;Duan, Minxian;Zhang, Birong;Shi, Wenbin;Yan, Suyun;Li, Xuejia;Long, Chunrui;Liu, Hongming;Guo, Lina;Zhou, Xianyan;Yang, Jing;Zhang, Hanyao
关键词：Primary metabolites; Secondary metabolites; Lignin accumulation; Cross-pollination; Juice sacs
-
Virulence and Diversity of Puccinia striiformis f. sp. tritici Populations in Northwestern and Southwestern Oversummering Regions of China
作者：Wang, Jie;Liu, Xinyun;Ma, Mengjie;Jin, Yongjin;Fu, Liyao;Huang, Lili;Kang, Zhensheng;Zhan, Gangming;Wang, Jie;Liu, Xinyun;Jin, Yongjin;Fu, Liyao;Li, Mingju;Huang, Lili;Kang, Zhensheng;Zhan, Gangming;Wang, Jie;Liu, Xinyun;Ma, Mengjie;Jin, Yongjin;Fu, Liyao;Huang, Lili;Kang, Zhensheng;Zhan, Gangming;Li, Mingju
关键词：diversity; oversummering regions;
Puccinia striiformis f. sp.tritici ; triadimefon; wheat stripe rust -
Genome-Wide Identification of the bHLH Gene Family in Kiwifruit (Actinidia chinensis) and the Responses of AcbHLH84 and AcbHLH97 Under Drought Stress
作者：Zhao, Ke;Yin, Tuo;Liu, Xiaozhen;Zhang, Hanyao;Xu, Rong;Chen, Xia;Ding, Renzhan
关键词：
Actinidia chinensis ;bHLH gene family; JA; transcriptome; metabolome
