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Anti-TMV activity and functional mechanisms of two sesquiterpenoids isolated from Tithonia diversifolia

文献类型： 外文期刊

作者： Zhao, Lihua 1 ; Dong, Jiahong 2 ; Hu, Zhonghui 3 ; Li, Shunlin 3 ; Su, Xiaoxia 2 ; Zhang, Jie 2 ; Yin, Yueyan 2 ; Xu, Tao 2 ;

作者机构： 1.Yunnan Agr Univ, Coll Plant Protect, Kunming 650201, Yunnan, Peoples R China

2.Yunnan Acad Agr Sci, Inst Biotechnol & Germplasm Resources, Kunming 650204, Yunnan, Peoples R China

3.Chinese Acad Sci, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R China

关键词： Curative activities;Sesquiterpene;Half-leaf method;Bio-pesticides

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：3.963； 五年影响因子：4.454 ）

ISSN： 0048-3575

年卷期： 2017 年 140 卷

页码：

收录情况： SCI

摘要： Unlike chemical pesticides, antiviral plants are biodegradable, replenishable and safe. In this study, 14 sesquiterpene compounds from Tithonia diversifolia were tested for their activities against Tobacco mosaic virus (TMV) using the half-leaf method. Tagitinin C (Ses-2) and 1 beta-methoxydiversifolin-3-0-methyl ether (Ses-5) were found to have in vivo curative activities of 62.86% and 60.27% respectively, at concentrations of 100 mu g/mL, respectively. In contrast, the in vivo curative inhibition rate of control agent ningnanmycin was 52.48%. Indirect enzyme-linked immunosorbent assay (ID-ELISA) also verified Ses-2 and Ses-5 had higher inhibition activities than the control agent ningnanmycin. Additionally, qRT-PCR showed that both Ses-2 and Ses-5 can partly inhibit the expression of CP and RdRp, two genes that play key roles in TMV infection. When TMV started to systemically spread, Ses-2 inhibited CP expression while Ses-5 inhibited RdRp expression. These results suggest that the two bio-agents have anti-TMV activities and may be used as bio-pesticides to control the plant virus. 2017 The Authors. Published by Elsevier Inc.

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