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Characterization of PEBP-like Genes and Function of Capebp1 and Capebp5 in Fruiting Body Regeneration in Cyclocybe aegerita

文献类型： 外文期刊

作者： Tao, Nan 1 ; Cheng, Bopu 1 ; Ma, Yuanhao 1 ; Liu, Ping 1 ; Chai, Hongmei 1 ; Zhao, Yongchang 1 ; Chen, Weimin 1 ;

作者机构： 1.Yunnan Acad Agr Sci, Biotechnol & Germplasm Resources Inst, Kunming 650223, Peoples R China

2.Yunnan Prov Key Lab Agr Biotechnol, Kunming 650223, Peoples R China

3.Minist Agr, Key Lab Southwestern Crop Gene Resources & Germpla, Kunming 650223, Peoples R China

关键词： Cyclocybe aegerita; fruiting body development; lamella redifferentiation; overexpression; PEBP; RNA interference

期刊名称：JOURNAL OF FUNGI （ 影响因子：4.0； 五年影响因子：4.5 ）

ISSN：

年卷期： 2024 年 10 卷 8 期

页码：

收录情况： SCI

摘要： Phosphatidylethanolamine-binding proteins (PEBPs) play a crucial role in the growth and development of various organisms. Due to the low sequence similarity compared to plants, humans, and animals, the study of pebp genes in fungi has not received significant attention. The redifferentiation of fruiting bodies is exceedingly rare in fungal development. Hitherto, only a few studies have identified the Capebp2 gene as being associated with this phenomenon in Cyclocybe aegerita. Thus, exploring the role of pebp genes in fruiting body development is imperative. In the present study, four Capebp genes (Capebp1, Capebp3, Capebp4, and Capebp5) were cloned from the AC0007 strain of C. aegerita based on genome sequencing and gene prediction. The findings indicate that the pebp family, in C. aegerita, comprises a total of five genes. Moreover, the sequence similarity was low across the five CAPEBP protein sequences in C. aegerita, and only a few conserved sequences, such as HRY and RHF, were identical. Expression analyses revealed that, similarly to Capebp2, the four Capebp genes exhibit significantly higher expression levels in the fruiting bodies than in the mycelium. Furthermore, overexpressed and RNA interference Capebp1 or Capebp5 transformants were analyzed. The results demonstrate that overexpression of Capebp1 or Capebp5 could induce the regeneration of the lamella or fruiting body, whereas the knockdown of Capebp1 or Capebp5 could lead to the accelerated aging of fruiting bodies. These findings highlight a significant role of Capebp genes in the generation of C. aegerita fruiting bodies and provide a foundation for further exploration into their involvement in basidiomycete growth and development.

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