Synergism Among the Four Tobacco Bushy Top Disease Casual Agents in Symptom Induction and Aphid Transmission
文献类型: 外文期刊
作者: Chen, Xiaojiao 1 ; Luo, Hengming 1 ; Zhang, Jingyi 1 ; Ma, Yan 1 ; Li, Kehua 1 ; Xiong, Feng 1 ; Yang, Yahui 1 ; Yang, Jiazhen 2 ; Lan, Pingxiu 1 ; Wei, Taiyun 3 ; Xu, Yi 4 ; Chen, Hairu 1 ; Li, Fan 1 ;
作者机构: 1.Yunnan Agr Univ, State Key Lab Conservat & Utilizat Bioresources Yu, Kunming, Peoples R China
2.Yunnan Acad Agr Sci, Inst Biotechnol & Germplasm Resources, Key Lab Agr Biotechnol Yunnan Prov, Kunming, Peoples R China
3.Fujian Agr & Forestry Univ, Inst Plant Virol, Fuzhou, Peoples R China
4.Nanjing Agr Univ, Dept Plant Pathol, Nanjing, Peoples R China
关键词: tobacco bushy top virus; tobacco vein distorting virus; TBTVsatRNA; TVDV-associated RNA; synergism; aphid transmission
期刊名称:FRONTIERS IN MICROBIOLOGY ( 影响因子:6.064; 五年影响因子:6.843 )
ISSN:
年卷期: 2022 年 13 卷
页码:
收录情况: SCI
摘要: Tobacco bushy top disease (TBTD), caused by multiple pathogens including tobacco bushy top virus (TBTV), tobacco vein distorting virus (TVDV), TBTV satellite RNA (TBTVsatRNA), and TVDV-associated RNA (TVDVaRNA), is a destructive disease in tobacco fields. To date, how these causal agents are co-transmitted by aphid vectors in field and their roles in disease symptom induction remain largely unknown, due mainly to the lack of purified causal agents. In this study, we have constructed four full-length infectious clones, representing the Yunnan Kunming isolates of TVDV, TBTV, TBTVsatRNA, and TVDVaRNA (TVDV-YK, TBTV-YK, TBTVsatRNA-YK, and TVDVaRNA-YK), respectively. Co-inoculation of these four causal agents to tobacco K326 plants caused typical TBTD symptoms, including smaller leaves, necrosis, and plant stunting. In addition, inoculation of tobacco K326 plants with TBTV alone caused necrosis in systemic leaves by 7 dpi. Tobacco K326 and Nicotiana benthamiana plants infected by single virus or multiple viruses showed very different disease symptoms at various dpi. RT-PCR results indicated that co-infection of TVDVaRNA-YK could increase TVDV-YK or TBTV-YK accumulation in N. benthamiana plants, suggesting that TVDVaRNA-YK can facilitate TVDV-YK and TBTV-YK replication and/or movement in the infected plants. Aphid transmission assays showed that the successful transmission of TBTV-YK, TBTVsatRNA-YK, and TVDVaRNA-YK by Myzus persicae depended on the presence of TVDV-YK, while the presence of TBTVsatRNA-YK increased the aphid transmission efficiency of TBTV and TVDV. We consider that these four new infectious clones will allow us to further dissect the roles of these four causal agents in TBTD induction as well as aphid transmission.
- 相关文献
作者其他论文 更多>>
-
Health-promoting compounds in Amomum villosum Lour and Amomum tsao-ko : Fruit essential oil exhibiting great potential for human health
作者:Imran, Sehrish;Bibi, Yamin;Yang, Li-E;Yang, Jiazhen;Yang, Xiaomeng;Pu, Xiaoying;Li, Xia;Zeng, Yawen;Qayyum, Abdul;He, Wei
关键词:Zingiberaceae; Amomum; Camphor; Borneol; Nutraceutical; Neuroprotective
-
Genetic mechanisms of petal morphogenesis in Eustoma grandiflorum
作者:Liang, Yuwei;Fang, Huiyi;Wang, Qi;Sun, Fuhui;Bi, Bo;Zhang, Liangsheng;Zhao, Peng;Liu, Bin;Sun, Dan;Ruan, Jiwei;Jin, Chunlian;Li, Fan;Zhao, Peng;Liu, Bin;Zhang, Liangsheng;Bi, Bo
关键词:Eustoma grandiflorum; petal morphogenesis; transcriptional analysis; WGCNA; qRT-PCR; YAB3; LFY
-
Physiological Mechanisms by Which the Functional Ingredients in Beer Impact Human Health
作者:Zeng, Yawen;Li, Xia;Yang, Li'e;Pu, Xiaoying;Yang, Xiaomeng;Yang, Tao;Ahmed, Hafiz Ghulam Muhu-Din;Yang, Jiazhen
关键词:functional ingredients; actional mechanism; barley grains and grass; beer; malt; hops; functional foods; chronic diseases; human
-
Studies of the pollen characteristics and the taxonomic significance of Impatiens from the Yunnan-Guizhou Plateau
作者:He, Hai-hao;Ma, Ming-lan;Chen, Xin-yi;Li, Xin-yi;Li, Fan;Zhao, Qiu-yan;He, Xiao-shuai;Tan, Yi;Huang, Hai-quan;Huang, Mei-juan;Qu, Su-ping
关键词:Impatiens; micromorphology; palynology; pollen; SEM
-
NLRs derepress MED10b-and MED7-mediated repression of jasmonate- dependent transcription to activate immunity
作者:Wu, Qian;Tong, Cong;Chen, Zhengqiang;Huang, Shen;Hong, Hao;Li, Jia;Feng, Mingfeng;Wang, Huiyuan;Xu, Min;Yan, Yuling;Cui, Hongmin;Shen, Danyu;Ai, Gan;Xu, Yi;Dong, Suomeng;Wang, Xuan;Zhu, Min;Tao, Xiaorong;Zhao, Xiaohui;Wang, Huiyuan;Li, Junming;Zhang, Hui;Huang, Changjun;Zhang, Zhongkai;Dinesh-Kumar, Savithramma P.;Dinesh-Kumar, Savithramma P.
关键词:Mediator complex; NLRs; Sw-5b; effector-triggered immunity
-
Discrimination and Evaluation of Wild Paris Using UHPLC-QTOF-MS and FT-IR Spectroscopy in Combination with Multivariable Analysis
作者:Yang, Yuangui;Xu, Hongbo;Tang, Zhishu;Yang, Yuangui;Xu, Hongbo;Tang, Zhishu;Li, Fan;Tang, Zhishu;Wang, Yuanzhong
关键词:
-
Ectopic Expression of PgF3 ' 5 ' H in Commercial Gypsophila paniculata Cultivar through Optimized Agrobacterium-Mediated Transformation
作者:Jin, Chunlian;Sun, Dan;Ma, Lulin;Mo, Xijun;Yang, Chunmei;Li, Fan
关键词:Gypsophila paniculata; regeneration; genetic transformation; commercial cultivar; PgF3 ' 5 ' H