您好，欢迎访问云南省农业科学院 机构知识库！

Biochemical characteristics of a novel protease from the basidiomycete Amanita virgineoides

文献类型： 外文期刊

作者： Yuan, Xianghe 1 ; Zhu, Mengjuan 3 ; Tian, Guoting 4 ; Zhao, Yongchang 4 ; Zhao, Liyan 5 ; Ng, Tzi Bun 6 ; Wang, Hexian 1 ;

作者机构： 1.China Agr Univ, State Key Lab Agrobiotechnol, Yuanmingyuan West Rd 2, Beijing 100193, Peoples R China

2.China Agr Univ, Dept Microbiol, Yuanmingyuan West Rd 2, Beijing 100193, Peoples R China

3.Shandong Agr Univ, Dept Fungal Resource, Tai An, Shandong, Peoples R China

4.Yunnan Acad Agr Sci, Inst Biotechnol & Germplasm Resource, Kunming, Yunnan, Peoples R China

5.Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing, Jiangsu, Peoples R China

6.Chinese Univ Hong Kong, Sch Biomed Sci, Fac Med, Hong Kong, Hong Kong, Peoples R China

关键词： Amanita virgineoides;properties;protease;purification;peptide sequences

期刊名称：BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY （ 影响因子：2.431； 五年影响因子：2.124 ）

ISSN： 0885-4513

年卷期： 2017 年 64 卷 4 期

页码：

收录情况： SCI

摘要： The characterization of a novel protease from Amanita virgineoides is described. The A. virgineoides protease was purified to homogeneity using Q-Sepharose, carboxymethyl-cellulose, diethylaminoethyl-cellulose, and a gel filtration step on Superdex 75. The molecular mass of the purified protease was estimated to be 16.6kDa. The protease was purified 32.1-fold, and its specific activity was 301.4 U/mg. The optimum pH was 4.0, and the optimum temperature was 50 degrees C. Kinetic constants (K-m, V-max) were determined under the optimum reaction conditions, with K-m and V-max, being 3.74mg/mL and 9.98 g mL(-1) Min(-1), respectively. The activity of the protease was curtailed by Cu2+, Pb2+, Fe3+, Cd2+, and Hg2+ ions but enhanced by Mg2+, Ca2+, and K+ ions at low concentrations. The protease activity was adversely affected by ethylene diamine tetraacetic acid, suggesting that it is a metalloprotease. Four peptide sequences were obtained from liquid chromatography-tandem mass spectrometry, including KQALSGIR, TIAMDGTEGLVR, VALTGLTVAEYFR, and AGAGSATLSMAYAGAR, which showed 86%, 64%, 60%, and 75% identity with peptides of Hypsizygus marmoreus, Dacryopinax sp. DJM-731 SS1, Trametes versicolor FP-101664 SS1, and Paxillus involutus ATCC 200175, respectively. The newly isolated protease showed good hydrolytic activity and biochemical characteristics, which expanded the knowledge of biologically active proteins and provided further insight on this poisonous fungus. (C) 2016 International Union of Biochemistry and Molecular Biology, Inc.

  • 相关文献

[1]Purification and Characterization of a Novel Serine Protease from the Fruiting Bodies of a Rare Edible Medicinal Mushroom, Lyophyllum shimeji (Agaricomycetes). Geng, Xueran,Te, Rigen,Wang, Hexiang,Geng, Xueran,Te, Rigen,Wang, Hexiang,Tian, Guoting,Zhao, Yongchang,Zhao, Liyan,Ng, Tzi Bun.

[2]Purification and characterization of a novel metalloprotease from fruiting bodies of Oudemansiella radicata. Geng, Xueran,Geng, Xueran,Te, Rigen,Wang, Hexiang,Geng, Xueran,Te, Rigen,Wang, Hexiang,Tian, Guoting,Zhao, Yongchang,Zhao, Liyan,Ng, Tzi Bun. 2017

[3]A novel alkaline protease with antiproliferative activity from fresh fruiting bodies of the toxic wild mushroom Amanita farinosa. Sun, Jian,Wang, Hexiang,Sun, Jian,Wang, Hexiang,Zhao, Yongchang,Chai, Hongmei,Ng, Tzi Bun.

[4]Purification and characterization of a novel laccase from the mushroom pleurotus nebrodensis. Wang, He-Xiang,Wang, He-Xiang,Ng, Tzi Bun,Zhang, Guo-Qing,Tian, Guo-Ting.

[5]A novel lectin with highly potent antiproliferative and HIV-1 reverse transcriptase inhibitory activities from the edible wild mushroom Russula delica. Zhao, Shuang,Zhao, Yongchang,Li, Shuhong,Ng, Tzi Bun,Zhao, Shuang,Zhao, Jingkun,Zhang, Guoqing,Wang, Hexiang.

[6]An antiproliferative ribonuclease from fruiting bodies of the wild mushroom Russula delica. Zhao Shuang,Zhang, Guo Qing,Wang, He Xiang,Zhao Shuang,Zhang, Guo Qing,Wang, He Xiang,Zhao Shuang,Zhao, Yong Chang,Li, Shu Hong,Ng, Tzi Bun.

[7]A novel ribonuclease with potent HIV-1 reverse transcriptase inhibitory activity from cultured mushroom Schizophyllum commune. Zhang, Guo-Qing,Wang, He-Xiang,Zhang, Guo-Qing,Wang, He-Xiang,Zhao, Yong-Chang,Zhang, Guo-Qing,Ng, Tzi-Bun.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：云南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台