Isolation and identification of a new Bombyx mori nucleopolyhedrovirus strain isolated from Yunnan, China
文献类型: 外文期刊
作者: Tang, Fenfen 1 ; Zhang, Yonghong 1 ; Shao, Yulan 1 ; Zhu, Feng 1 ; Huang, Ping 1 ; Bai, Xingrong 1 ;
作者机构: 1.Yunnan Acad Agr Sci, Inst Sericulture & Apiculture, Mengzi 661101, Yunnan, Peoples R China
2.Yunnan Agr Univ Kunming, Coll Plant Protect, Kunming 650201, Peoples R China
关键词: Bombyx mori nucleopolyhedrovirus;strain identification;polh;bro
期刊名称:SCIENCEASIA ( 影响因子:0.615; 五年影响因子:0.708 )
ISSN: 1513-1874
年卷期: 2017 年 43 卷 1 期
页码:
收录情况: SCI
摘要: Bombyx mori nucleopolyhedrovirus (BmNPV) is a silkworm pathogen that causes serious economic losses in sericulture. In this study, a new BmNPV strain, designated as BmNPV-YN1, from Yunnan (China), was isolated and identified through molecular identification. Sequence analysis results revealed that polyhedrin (polh) presented the highest identity among various BmNPVs; this gene indicated that BmNPV-YN1 is a BmNPV strain. The baculovirus repeated-ORF (bro) genes of this isolate were different from those of six BmNPV strains (BmNPV-T3, BmNPV-ZJ, BmNPV-GX, BmNPV-India, BmNPV-Cubic, and BmNPV-Brazilian). The BmNPV-YN1 bro family includes bro-a, bro-b, bro-c, bro-d, and bro-e, which are similar to those of BmNPV-T3. Sequence alignment results further showed the differences in the composition and sequences of the bro family members among various strains. The bro-d sequences were also phylogenetically analysed. Results demonstrate that BmNPV-YN1, BmNPV-Cubic, and BmNPV-India are closely related. Despite this close relationship, BmNPV-YN1 belongs to a distinct cluster. The BmNPV DNA was subjected to restriction endonuclease analysis and the obtained results provided additional evidence supporting the identity of the novel BmNPV-YN1 strain. Indeed, BmNPV-YN1 isolated from Yunnan is a new BmNPV strain. The proposed method can be used to rapidly and accurately detect and identify BmNPVs strains.
- 相关文献
作者其他论文 更多>>
-
Integrated transcriptomics and metabolomics reveal the mechanisms of postharvest uneven degreening of green lemon
作者:Zhou, Xianyan;Zhang, Birong;Duan, Minxian;Yan, Suyun;Shi, Wenbin;Yue, Jianqiang;Guo, Lina;Du, Yuxia;Gao, Junyan;Zhu, Zhifeng;Xu, Rangwei;Zhu, Feng;Cheng, Yunjiang;Zhang, Hanyao
关键词:Green lemon; Color; Degreen; Pigment metabolism; Hormones; Gene
-
The complete mitochondrial genome and phylogenetic analysis of Pealius mori (Hemiptera: Aleyrodidae)
作者:Zhang, Yonghong;Chai, Jianping;Yang, Zhenguo;Luo, Jiafu
关键词:Pealius mori; aleyrodidae; mitochondrion; phylogenetic tree
-
Genetic Analysis of a Collection of Rice Germplasm (Oryza sativa L.) through High-Density SNP Array Provides Useful Information for Further Breeding Practices
作者:Huang, Ping;Gu, Qiongyao;Li, Huahui;Wu, Zhigang;Liu, Weihua;Zhu, Zhenhua;Yuan, Pingrong;Kou, Shuyan;Hu, Yong;Duan, Liu;Liu, Li;Zhou, Ying;Luo, Hanyu
关键词:genetic diversity; rice; SNP; local variety
-
Identification and subcellular localization of NbIAP in the microsporidian Nosema bombycis
作者:Zhu, Feng;Qin, Xingrong;Liu, Qunyan;Li, Hui;Ming, Dongfeng;Zhu, Feng;Bai, Xingrong;Xiao, Shengyan
关键词:Nosema bombycis; Inhibitor of apoptosis protein; Immunolocalization; RNAi
-
CCT8 colocalizes with actin and beta-tubulin: Insight into its involvement in the cytoskeleton formation of the intracellular parasite Nosema bombycis
作者:Qi, Jingru;Chen, Ying;He, Ping;Shang, Ruisha;Sun, Fuzhen;Wang, Qiang;Zhang, Yiling;Tang, Xudong;Shen, Zhongyuan;Zhu, Feng;Shao, Li;Li, Jicheng;Wang, Qiang;Zhang, Yiling;Tang, Xudong;Shen, Zhongyuan;Zhu, Feng
关键词:Nosema bombycis; Chaperonin; CCT; Immunolocalization; RNAi
-
First Report on Megaselia scalaris Loew (Diptera: Phoridae) Infestation of the Invasive Pest Spodoptera frugiperda Smith (Lepidoptera: Noctuidae) in China
作者:Tang, Yunlin;Li, Qingyan;Xiang, Li;Gu, Ruocheng;Wu, Yanyan;Li, Tian;Wei, Junhong;Pan, Guoqing;Zhou, Zeyang;Tang, Yunlin;Li, Qingyan;Gu, Ruocheng;Wu, Yanyan;Li, Tian;Wei, Junhong;Pan, Guoqing;Zhou, Zeyang;Tang, Yunlin;Li, Qingyan;Gu, Ruocheng;Wu, Yanyan;Li, Tian;Wei, Junhong;Pan, Guoqing;Zhou, Zeyang;Xiang, Li;Zhang, Yonghong;Bai, Xingrong;Niu, Xiaohui;Zhou, Zeyang
关键词:Spodoptera frugiperda; invasive pest; natural enemies; Megaselia scalaris; pest management
-
Integration of metabolome, histochemistry and transcriptome analysis provides insights into lignin accumulation in oleocellosis-damaged flavedo of citrus fruit
作者:Zhou, Xianyan;Yang, Hongbin;Zhu, Feng;Xu, Rangwei;Deng, Xiuxin;Cheng, Yunjiang;Zhou, Xianyan;Yue, Jianqiang;Zhu, Chunhua;Li, Jinxue;Gao, Junyan;Zhou, Dongguo;Zhou, Xianyan;Yang, Hongbin;Zhu, Feng;Xu, Rangwei;Deng, Xiuxin;Cheng, Yunjiang
关键词:Green lemon; Oleocellosis disorder; Primary metabolites; Secondary metabolites; Lignification; Pathogen resistance