High Performance Liquid Chromatography Fingerprinting of Flavonoids Between Parents and Generations in Barley Grain
文献类型： 外文期刊
作者： Yang, Tao 1 ; Duan, Chengli 2 ; Zeng, Yawen 1 ; Du, Juan 1 ; Yang, Shuming 1 ; Pu, Xiaoying 1 ; Yang, Shengchao 2 ;
作者机构： 1.Yunnan Acad Agr Sci, Agr Biotechnol Key Lab Yunnan Prov, Biotechnol & Genet Germplasm Inst, Kunming 650205, Peoples R China
2.Yunnan Agr Univ, Coll Agron & Biotechnol, Kunming 650201, Peoples R China
3.Zhejiang Forestry Univ, Sch Forestry & Biotechnol, Hangzhou 311300, Zhejiang, Peoples R China
关键词： Barley;HPLC;Flavonoids;Fingerprinting
期刊名称：JOURNAL OF BIOBASED MATERIALS AND BIOENERGY （ 影响因子：0.708； 五年影响因子：0.739 ）
ISSN： 1556-6560
年卷期： 2017 年 11 卷 1 期
页码：
收录情况： SCI
摘要： Barley is a self-pollination of plants, species rich in resources with flavonoids which have a wide range of biological activity. In this study, the fingerprints of flavonoids are studied between Parents and F-1 generations in barley grain. The HPLC were determined on a C18 column (4.6 mm x 250 mm, 5 micron) eluted with 0.1% acetic acid aqueous solution (A) and acetonitrile (B) as mobile phases in a linear gradient elution, and with the flowrate of 0.8 mL/min. The detection wavelength was set at 280 nm. As a result, the different variety of barley and its generations showed different HPLC fingerprints, different types of flavonoids express differences between parents and F-1 generations. Not only the group of high x high combinations but that of high x low combinations can produce heterosis.
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