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Study on the identification of Polygonatum kingianum and its adulterants by PCR amplification of specific alleles

文献类型： 外文期刊

作者： Li, Yanying 1 ; Zhu, Xinyan 1 ; Li, Yiguo 3 ; Yang, Yan 1 ; Shi, Yana 1 ; Hu, Xiaobin 1 ; Wang, Jiajing 1 ; Ji, Pengzhang 1 ;

作者机构： 1.Yunnan Acad Agr Sci, Inst Med Plant, Kunming 650223, Yunnan, Peoples R China

2.Yunnan Agr Univ, Coll Food Sci & Technol, Kunming 650092, Yunnan, Peoples R China

3.Kunming Univ Sci & Technol, Fac Sci & Technol, Kunming 650500, Yunnan, Peoples R China

期刊名称：2016 INTERNATIONAL CONFERENCE ON MEDICINE SCIENCES AND BIOENGINEERING (ICMSB2016)

ISSN： 2117-4458

年卷期： 2017 年 8 卷

页码：

收录情况： SCI

摘要： Using the allele-specific diagnostic PCR for the rapid and efficient identification of Chinese herbal medicines and their adulterants. In this paper, we analyze the research object of P. kingianum and its adulterants. The total DNA of the samples to be identified was extracted, and the PCR was amplified by using universal primers. After the homology comparison, the specific sites were found out by using BioEdit software, and the specific PCR primers were designed by Premier primer 5.0 software, and the samples were amplified by the primers. The 331bp bands were amplified by P. kingianum of the specific primers, but the results of their adulterants were not amplified. The results show that the method can provide guidance for the allele-specific diagnostic PCR for quick and easy to select P. kingianum, which is not affected by environmental factors, affecting the growth period of the plant, were identified directly from the molecular level of DNA. This method having important application value in accurate introduction and clinical application can provide a model for other identification.

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