文献类型： 外文期刊

作者： Yang Ni-na ¹ ; Zhang Ji ³ ; Zhao Yan-li ³ ; Wang Yuan-zhong ³ ; Zhao Ying-hong ¹ ;

作者机构： 1.Dai Hosp Xishuangbanna Dai Autonomous Prefecture, Xishuangbanna 666100, Peoples R China

2.Yunnan Univ TCM, Coll TCM, Kunming 650500, Peoples R China

3.Yunnan Acad Agr Sci, Inst Med Plants, Kuming 650200, Peoples R China

关键词： UV-Vis;HPLC fingerprint;Dai medicine;Alstonia scholaris (L.) R. Br.;Harvest time;Distinguish;Quality estimation

期刊名称：SPECTROSCOPY AND SPECTRAL ANALYSIS （ 影响因子：0.589； 五年影响因子：0.504 ）

ISSN： 1000-0593

年卷期： 2016 年 36 卷 12 期

页码：

收录情况： SCI

摘要： UV-Vis and HPLC fingerprint of different harvest time of the leaves of Alstonia scholaris (L.) R. Br. were establish the for identification and quality evaluation to promote the development of Dai Medicine modernization. The optimal extraction condition was used to obtain UV - vis data of different harvest time which were deducted background and eight spot smooth, were collected to make the principal component analysis in SIMCA-P+11.5, identifying the samples quickly with the first three principal component three-dimensional diagram. The HPLC fingerprint were obtained with Agilent ZORBAX Eclipse XDB C18 (250x4.6 mm, 5 m) chromatographic column with the mobile phase of acetonitrile (B) - water (contain 0.1% formic acid) (A) for gradient elution (0 similar to 5 min, 5% B; 5 similar to 35 min, 5% B -> 26% B; 35 similar to 40 min, 26% B -> 56% B). The wavelength was set at 287 nm and the column temperature was maintained at 30 ->. The flow rate was 1.0 mLmin-1 and the injection volume was 7 L. The HPLC fingerprint of different harvest time of the leaves of Alstonia scholaris (L.) R. Br. was analysised by cluster analysis to quality evaluation. Research findings showing: (1) The UV-Vis spectrogram of different harvest time of the leaves of Alstonia scholaris (L.) R. Br. were divided into three parts according to the absorption peak position and amplitude of variation. The first was 235 to 400 nm, the second was 400 to 500 nm, and the third was 500 to 800 nm. In the first part, absorption peak were focused on 270, 287 and 325 nm, which can reflect the fingerprint character for the high absorbance and amplitude of variation. Absorption peak were distributed in 410 and 464 nm in the second part, absorbance and amplitude of variation were lower than the first part. There was a bigger absorption peak at 665 nm in the third part, but the absorbance had no difference. The UV-Vis data of different harvest time were gathered to make the principal component analysis, the result was that the samples of same month were concentrated distribution, but different month samples were dispersed distribution. (2) HPLC fingerprint were divided into three categories through hierarchical cluster analysis, 3, 4, 5 and 7 month were the first category, 6, 8, 9 month samples were second category, the others were third category. Chemical composition and content of the same category samples were similar, but the different category samples had a obvious difference, more important is that the third category samples content was the highest. Combining UV-Vis FP and HPLC FP can identify and evaluate quickly the samples of different harvest time of the leaves of Alstonia scholaris (L.) R. Br. The optimal harvest time of Alstonia scholaris (L.) R. Br. was from October to next February, which was the coldest season in the Dai calendar.