Genetic Diversity of Populations of Saccharum spontaneum with Different Ploidy Levels Using SSR Molecular Markers
文献类型： 外文期刊
作者： Liu, X. L. 1 ; Li, X. J. 2 ; Xu, C. H. 2 ; Lin, X. Q. 2 ; Deng, Z. H. 1 ;
作者机构： 1.Fujian Agr & Forestry Univ, Key Lab Sugarcane Biol & Genet Breeding, Minist Agr, Fuzhou 350002, Peoples R China
2.Yunnan Key Lab Sugarcane Genet Improvement, Kaiyuan 661600, Yunnan, Peoples R China
3.Yunnan Acad Agr Sci, Sugarcane Res Inst, Kaiyuan 661600, Yunnan, Peoples R China
关键词： Saccharum spontaneum;Polyploidy;Genetic diversity;SSR
期刊名称：SUGAR TECH （ 影响因子：1.591； 五年影响因子：1.688 ）
ISSN： 0972-1525
年卷期： 2016 年 18 卷 4 期
页码：
收录情况： SCI
摘要： Saccharum spontaneum L. plays a key role in the improvement of stress resistance and yield of sugarcane cultivars by serving as wild parent sources, especially lines of different ploidy. To better understand the genetic diversity and potential breeding value of S. spontaneum with different ploidy levels, 62 clones from four ploidy types (2n = 64, 72, 80, and 96) were screened using 30 pairs of SSR primers and with population genetics methods. The results showed that the decaploid and octaploid populations had a higher diversity than nonaploid and dodecaploid populations at 30 SSR loci with > 98 % percentage of polymorphic bands and > 0.92 polymorphic information content. Both the octaploid and decaploid populations shared more common bands with commonly used parents than the nonaploid or dodecaploid populations. The analysis of Nei's genetic distance and UPGMA tree indicates that the decaploid population had the closest genetic relationship with the octaploid population and that the two populations had a close genetic relationship with commonly used parents (controls). According to the population divergence analysis, the four different ploidy populations did not show an obvious genetic divergence (Gst) (0.0880), but clearly had large amounts of gene flow (Nm) (5.1840). Octaploid and decaploid populations appeared to have undergone more genetic exchange with commonly used parents, as indicated by the low Gst value (0.1254 and 0.1276) and high Nm value (3.4872 and 3.4186). These results may provide important insight into utilizing the diversity of clones with different ploidy levels in S. spontaneum for future sugarcane breeding.
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作者：Cai, Q.;Cai, Q.;Fan, Y. H.;Liu, X. L.;Cai, Q.;Huang, X. Q.;Aitken, K. S.;McIntyre, C. L.;Piperidis, G.;Jackson, P.
关键词：AFLPs;Erianthus arundinaceus;Genetic diversity;Sugarcane
