您好，欢迎访问云南省农业科学院 机构知识库！

Identification of three new isolates of Tomato spotted wilt virus from different hosts in China: molecular diversity, phylogenetic and recombination analyses

文献类型： 外文期刊

作者： Zhang, Zhenjia 2 ; Wang, Deya 2 ; Yu, Chengming 2 ; Wang, Zenghui 2 ; Dong, Jiahong 3 ; Shi, Kerong 1 ; Yuan, Xuefeng 2 ;

作者机构： 1.Shandong Agr Univ, Coll Anim Sci & Technol, Tai An 271018, Shandong, Peoples R China

2.Shandong Agr Univ, Coll Plant Protect, 61 Daizong St, Tai An 271018, Shandong, Peoples R China

3.Minist Agr, Key Lab Southwestern Crop Gene Resources & Germpl, Yunnan Prov Key Lab Agr Biotechnol, Yunnan Acad Agr Sci,Resources Inst, Kunming 650223, Peoples R China

关键词： Tomato spotted wilt virus;Phylogenetic analysis;Reassortment;Recombination

期刊名称：VIROLOGY JOURNAL （ 影响因子：4.099； 五年影响因子：3.719 ）

ISSN： 1743-422X

年卷期： 2016 年 13 卷

页码：

收录情况： SCI

摘要： Background: Destructive diseases caused by Tomato spotted wilt virus (TSWV) have been reported associated with many important plants worldwide. Recently, TSWV was reported to infect different hosts in China. It is of value to clone TSWV isolates from different hosts and examine diversity and evolution among different TSWV isolates in China as well as worldwide. Methods: RT-PCR was used to clone the full-length genome (L, M and S segments) of three new isolates of TSWV that infected different hosts (tobacco, red pepper and green pepper) in China. Identity of nucleotide and amino acid sequences among TSWV isolates were analyzed by DNAMAN. MEGA 5.0 was used to construct phylogenetic trees. RDP4 was used to detect recombination events during evolution of these isolates. Results: Whole-genome sequences of three new TSWV isolates in China were determined. Together with other available isolates, 29 RNA L, 62 RNA M and 66 RNA S of TSWV isolates were analyzed for molecular diversity, phylogenetic and recombination events. This analysis revealed that the entire TSWV genome, especially the M and S RNAs, had major variations in genomic size that mainly involve the A-U rich intergenic region (IGR). Phylogenetic analyses on TSWV isolates worldwide revealed evidence for frequent reassortments in the evolution of tripartite negative-sense RNA genome. Significant numbers of recombination events with apparent 5' regional preference were detected among TSWV isolates worldwide. Moreover, TSWV isolates with similar recombination events usually had closer relationships in phylogenetic trees. Conclusions: All five Chinese TSWV isolates including three TSWV isolates of this study and previously reported two isolates can be divided into two groups with different origins based on molecular diversity and phylogenetic analysis. During their evolution, both reassortment and recombination played roles. These results suggest that recombination could be an important mechanism in the evolution of multipartite RNA viruses, even negative-sense RNA viruses.

  • 相关文献

[1]Occurrence and diversity of Tomato spotted wilt virus isolates breaking the Tsw resistance gene of Capsicum chinense in Yunnan, southwest China. Jiang, L.,Huang, Y.,Sun, L.,Wang, B.,Zhu, M.,Li, J.,Tao, X.,Huang, C.,Liu, Y.,Li, F.,Liu, Y.,Dong, J.,Zhang, Z..

[2]Clustering and cellular distribution characteristics of virus particles of Tomato spotted wilt virus and Tomato zonate spot virus in different plant hosts. Zhang, Zhongkai,Wang, Xifeng,Zhang, Zhongkai,Zheng, Kuanyu,Dong, Jiahong,Fang, Qi,Hong, Jian. 2016

[3]Characterization of DNA beta associated with begomoviruses in China and evidence for co-evolution with their cognate viral DNA-A. Zhou, XP,Xie, Y,Tao, XR,Zhang, ZK,Li, ZH,Fauquet, CM.

[4]Sex at the origin: An Asian population of the rice blast fungus Magnaporthe oryzae reproduces sexually. Saleh, Dounia,Adreit, Henri,Milazzo, Joelle,Ravigne, Virginie,Bazin, Eric,Tharreau, Didier,Saleh, Dounia,Fournier, Elisabeth,Xu, Peng,Li, Chenguyn,Shen, Ying,Notteghem, Jean-Loup.

[5]Tobacco curly shoot virus isolated in Yunnan is a distinct species of Begomovirus. Xie, Y,Zhou, XP,Zhang, ZK,Qi, YJ. 2002

[6]GISH characterization of Erianthus arundinaceus chromosomes in three generations of sugarcane intergeneric hybrids. Piperidis, Nathalie,Piperidis, George,Chen, Jian-wen,Deng, Hai-hua,Wang, Li-Ping,Jackson, Phillip. 2010

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：云南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台