De Novo Assembly and Characterization of the Transcriptome of the Chinese Medicinal Herb, Gentiana rigescens
文献类型： 外文期刊
作者： Zhang, Xiaodong 1 ; Allan, Andrew C. 2 ; Li, Caixia 1 ; Wang, Yuanzhong 3 ; Yao, Qiuyang 4 ;
作者机构： 1.Yuxi Normal Univ, Coll Resources & Environm, Yuxi 653100, Peoples R China
2.Mt Albert Res Ctr, Plant & Food Res, Auckland 92169, New Zealand
3.Yunnan Acad Agr Sci, Inst Med Plants, Kunming 650223, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
关键词： Gentiana rigescens;gentiopicroside;regulation;transcriptome
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）
ISSN： 1422-0067
年卷期： 2015 年 16 卷 5 期
页码：
收录情况： SCI
摘要： Gentiana rigescens is an important medicinal herb in China. The main validated medicinal component gentiopicroside is synthesized in shoots, but is mainly found in the plant's roots. The gentiopicroside biosynthetic pathway and its regulatory control remain to be elucidated. Genome resources of gentian are limited. Next-generation sequencing (NGS) technologies can aid in supplying global gene expression profiles. In this study we present sequence and transcript abundance data for the root and leaf transcriptome of G. rigescens, obtained using the Illumina Hiseq2000. Over fifty million clean reads were obtained from leaf and root libraries. This yields 76,717 unigenes with an average length of 753 bp. Among these, 33,855 unigenes were identified as putative homologs of annotated sequences in public protein and nucleotide databases. Digital abundance analysis identified 3306 unigenes differentially enriched between leaf and root. Unigenes found in both tissues were categorized according to their putative functional categories. Of the differentially expressed genes, over 130 were annotated as related to terpenoid biosynthesis. This work is the first study of global transcriptome analyses in gentian. These sequences and putative functional data comprise a resource for future investigation of terpenoid biosynthesis in Gentianaceae species and annotation of the gentiopicroside biosynthetic pathway and its regulatory mechanisms.
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