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Identification of Dendrobium Varieties by Fourier Transform Infrared Spectroscopy Combined with Spectral Retrieval

文献类型： 外文期刊

作者： Liu Fei 1 ; Wang Yuan-zhong 2 ; Deng Xing-yan 3 ; Jin Hang 2 ; Yang Chun-yan 1 ;

作者机构： 1.Yuxi Normal Univ, Dept Phys, Yuxi 653100, Peoples R China

2.Yunnan Acad Agr Sci, Inst Med Plants, Kunming 650200, Peoples R China

3.Yunnan Univ Nationalities, Sch Chem & Biotechnol, Kunming 650500, Peoples R China

关键词： Infrared spectroscopy;Spectrum retrieval;Dendrobium;Identification

期刊名称：SPECTROSCOPY AND SPECTRAL ANALYSIS （ 影响因子：0.589； 五年影响因子：0.504 ）

ISSN： 1000-0593

年卷期： 2014 年 34 卷 6 期

页码：

收录情况： SCI

摘要： The infrared spectral of stems of 165 trees of 23 dendrobium varieties were obtained by means of Fourier transform infrared spectroscopy technique. The spectra show that the spectra of all the samples were similar, and the main components of stem of Dendrobium is cellulose. By the spectral professional software Omnic8. 0, three spectral databases were constructed. Lib01 includes of the average spectral of the first four trees of every variety, while Lib02 and Lib03 are constructed from the first-derivative spectra and the second-derivative spectra of average spectra, separately. The correlation search, the square difference retrieval and the square differential difference retrieval of the spectra are performed with the spectral database Lib01 in the specified range of 1 800 similar to 500 cm(-1), and the yield correct rate of 92.7%, 4.5% and 92.7%, respectively. The square differential difference retrieval of the first-derivative spectra and the second-derivative spectra is carried out with Lib02 and Lib03 in the same specified range 1 800 similar to 500 cm(-1), and shows correct rate of 93.9% for the former and 90.3% for the later. The results show that the first-derivative spectral retrieval of square differential difference algorithm is more suitabe for discerning dendrobium varieties, and FTIR combining with the spectral retrieval method can identify different varieties of Dendrobium, and the correlation retrieval, the square differential retrieval, the first-derivative spectra and second-derivative spectra retrieval in the specified spectral range are effective and simple way of distinguishing different varieties of dendrobium.

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