Cloning, Expression Feature, and Tissue Location of BmSCRBQ1, a Class B Scavenger Receptor in Bombyx mori (L.)
文献类型： 外文期刊
作者： Dong, Z. P. 1 ; Chai, C. L. 1 ; Dai, F. Y. 1 ; Pan, M. H. 1 ; Wang, W. 4 ; Lu, C. 1 ;
作者机构： 1.Southwest Univ, State Key Lab Silkworm Genome Biol, Chongqing 400716, Peoples R China
2.Yunnan Acad Agr Sci, Sericultural & Apicultural Res Inst, Mengzi 661101, Yunnan, Peoples R China
3.Southwest Univ, Coll Biotechnol, Chongqing 400716, Peoples R China
4.Southwest Univ, Coll Anim Sci & Technol, Chongqing 400716, Peoples R China
关键词： Bombyx mori;BmSCRBQ1;Cameo1;class B scavenger receptor;gene expression
期刊名称：ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA （ 影响因子：2.099； 五年影响因子：1.884 ）
ISSN： 0013-8746
年卷期： 2013 年 106 卷 4 期
页码：
收录情况： SCI
摘要： Class B scavenger receptors can combine with various kinds of ligands and are involved in lipid metabolism. They play an important role in a series of physiological processes, such as atherosclerosis formation or inhibition, congenital immune defense, apoptotic cell clearance, carotenoid transport, and pheromone transduction. In this study, based on a whole-genome database of the silkworm (Bombyx mori (L.)), the full-length CDS of BmSCRBQ1, a class B scavenger receptor gene, was cloned. BmSCRBQ1 consists of 10 exons and 9 introns, with an open reading frame of 1,482 bp encoding 493 amino acids. BmSCRBQ1 mRNA and protein were expressed in most tissues of larval B. mori strain Dazao and the silkworm cell lines BmN and BmE, suggesting that BmSCRBQ1 may have certain ubiquitous functions. Immunohistochemical staining showed that BmSCRBQ1 protein is located in the constitutive membranes or cell membranes of the testes, ovaries, fat body, blood cells, and the middle silk gland of larval B. mori Dazao, indicating that its function could be related to lipid metabolism; the binding, transport, and phagocytosis of exogenous materials; and signal transduction in these tissue types.
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