文献类型： 外文期刊
作者： Yang, J. 1 ; Dong, J. H. 2 ; Zhang, T. J. 2 ; Wang, R. 1 ; Luo, Z. P. 1 ; Luo, H. Y. 3 ; Zhang, Z. K. 2 ;
作者机构： 1.CNTC, Zhengzhou Tobacco Res Inst, Zhengzhou 450001, Peoples R China
2.Yunnan Acad Agr Sci, Biotechnol & Genet Germplasm Inst, Key Lab Agr Biotechnol Yunnan Prov, Kunming 650223, Peoples R China
3.Hongyun Honghe Tobacco Grp Ltd Co, Kunming 650202, Yunnan, Peoples R China
关键词： ChiVMV;sequencing;tobacco;polyprotein;potyvirus;phylogenetic tree
期刊名称：JOURNAL OF PLANT PATHOLOGY （ 影响因子：1.729； 五年影响因子：1.681 ）
ISSN： 1125-4653
年卷期： 2013 年 95 卷 1 期
页码：
收录情况： SCI
摘要： An isolate of Chilli veinal mottle virus (ChiVMV) was characterized, which infects tobacco (Nicotiana tabacum L.) in the Chinese province of Yunnan inducing mottling and necrotic spots on the leaves. Nine solanaceous crops inoculated mechanically proved susceptible to this viral isolate (ChiVMV-To) and reacted with symptoms including systemic mottling and leaf distortion. ChiVMV-To has filamentous particles ca. 760 nm long and a genome composed of 9,724 nucleotides (nts), excluding the poly(A) tail, comprised in a single open reading frame encoding a polypeptide 3,089 amino acids (aa) in size. The 5'- and 3'-untranslated regions (UTRs) are 168 and 286 nts in size, respectively. The complete genome sequence of ChiVMV-To shares 78.5 to 79.1% nt and 83.8 to 86.7% aa identities with those of six other ChiVMV chilli isolates reported previously. Phylogenetic analysis based on the polyprotein sequences of other potyviruses suggests ChiVMV-To to be most closely related to Wild tomato mosaic virus (WTMV). This is the first complete genome sequence characterization of ChiVMV isolated from tobacco in China.
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