A novel pig gene, CPNE1, differentially expressed in the muscle tissues from Wujin pigs and Large White pigs
文献类型： 外文期刊
作者： Pu Shifei 2 ; Kong Chuisi 3 ; Liu Yonggang 1 ;
作者机构： 1.Yunnan Agr Univ, Coll Anim Sci & Technol, Kunming 650201, Peoples R China
2.Yunnan Vocat & Tech Coll Agr, Dept Anim Husb & Vet, Kunming 650201, Peoples R China
3.Yunnan Acad Agr Sci, Inst Agr Environm & Resources, Kunming 650205, Peoples R China
关键词： CPN1;differential display;muscle;mRNA;pig
期刊名称：ANIMAL SCIENCE PAPERS AND REPORTS （ 影响因子：1.078； 五年影响因子：1.097 ）
ISSN： 0860-4037
年卷期： 2012 年 30 卷 2 期
页码：
收录情况： SCI
摘要： The mRNA differential display technique was performed to investigate gene expression differences in the longissimus dorsi muscle from Wujin and Large White pigs. A fragment of one differentially expressed gene was isolated and sequenced. A complete cDNA sequence of the gene was obtained using the rapid amplification of cDNA ends (RACE) method. The open reading frame of this gene encodes a protein of 537 amino acids, which is homologous with the Copine I (CPNE1) of eight species: greater horseshoe bat (95%), mouse (92%), rat (93%), cattle (95%), sheep (95%), rabbit (94%), human (95%) and dog (94%). This newly identified gene was respectively defined as the swine CPNE1 gene and had been assigned CeneID: 100233174. The phylogenetic analysis revealed that the swine CPNE1 gene has a closer genetic relationship with the CPNE1 gene of a dog. The tissue expression analysis indicated that the swine CPNE1 gene has a broad tissue distribution. The experiment described is the first to establish the primary foundation for further research on the swine CPNE1 gene.
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