文献类型： 外文期刊

作者： Chang, Wen-Jun ² ; Su, Huo-Sheng ⁴ ; Li, Wei-Jing ² ; Zhang, Zhi-Li ¹ ;

作者机构： 1.Hainan Acad Agr Sci, Haikou 571000, Peoples R China

2.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 571101, Peoples R China

3.Hainan Univ, Coll Agr, Haikou 571101, Peoples R China

4.Yunnan Acad Agr Sci, Sugarcane Res Inst, Kaiyuan 661600, Peoples R China

关键词： calcium-dependent protein kinase;in silico cloning;heat stress;phytohormone;tomato

期刊名称：BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY （ 影响因子：2.043； 五年影响因子：2.017 ）

ISSN： 0916-8451

年卷期： 2009 年 73 卷 11 期

页码：

收录情况： SCI

摘要： A full-length cDNA LeCPK2 (GenBank GQ205414) from tomato (Solanum lycopersicum) encoding a calcium-dependent protein kinase (CDPK) was cloned by in silico cloning using NtCPK5 (AY971376) as a virtual probe. The deduced amino acid sequence of LeCPK2 contained the kinase, autoinhibitory, and calmodulin-like domains typical of CDPKs. Expression profiling indicated that LeCPK2 expressed predominantly in flowers and responded divergently to heat and cold stress, in which obvious mRNA accumulation was detected at 4 h under 42 degrees C stress, but no change in LeCPK2 mRNA levels was observed in 6 h at 4 degrees C. Mechanical wounding and phytohormones including ethylene, methyl jasmonate, and salicylic acid were also observed to arouse the expression of LeCPK2 in a similar pattern. mRNA accumulation was enhanced at 30 min and reached a maximum at 3 h, followed by a decrease to the normal level. All the results suggest that LeCPK2 is a novel versatile isoform of tomato CDPKs.