文献类型： 外文期刊

作者： Liu Hong-Cheng ¹ ; Zou Yan-Hong ¹ ; Li Qi-Wan ¹ ; Zhu, Er ¹ ;

作者机构： 1.Yunnan Acad Agr Sci, Qual Standard & Testing Technol Inst, Minist Agr, Supervis & Testing Ctr Farm Prod Qual, Kunming 650223, Peoples R China

关键词： milk;estrogen;hexestrol;diethylstilbestrol;dienestrol;high performance liquid chromatography

期刊名称：CHINESE JOURNAL OF ANALYTICAL CHEMISTRY （ 影响因子：1.134； 五年影响因子：0.909 ）

ISSN： 0253-3820

年卷期： 2008 年 36 卷 2 期

页码：

收录情况： SCI

摘要： A multiresidues determination method was developed for the extraction of diethylstilbestrol (DES), hexestrol (HEX) and dienestrol (DE) in milk by matrix solid phase diverse techique (MSPD) and separation by HPLC. The retention behavior of estrogen in C-18, Florisil and Ethylenediamine-N-propyl (PSA), the composition of eluent and the chromatographic conditions for the separation of three estrogens were stadied. The three estrogens were better separated by Xterra C-18 (250 mm x 4.6 root, 5 mu m) column. The average recoveries of the determination were 84.1% - 93.5%, the relative standard deviations (RSD) were 3.5% - 7.8%. The limit of detection(LOD) was 0.004 mg/kg for DES, 0.004 mg/kg for HEX and 0.006 mg/kg for DE. The limit of quantity (LOQ) was 0.01 mg/kg for DES, 0.01 mg/kg for HEX and 0.02 mg/kg for DE. The real sample analysis shows that this method can be used for the sensitive and accurate determination of three estrogens residues in milk samples.