文献类型： 外文期刊

作者： Budahn, H. ¹ ; Peterka, H. ¹ ; Schrader, O. ¹ ; Zhang, S. ² ;

作者机构： 1.Inst Hort Crops, Fed Ctr Breeding Res Cultivated Plants, Neuer Weg 22-23, D-06484 Quedlinburg, Germany

2.Yunnan Acad Agr Sci, Biotechnol Res Inst, Kunming, Peoples R China

关键词： wide hybridization;Heterodera schachtii;FISH;RAPD

期刊名称：PROCEEDINGS OF THE JOINT MEETING OF THE FOURTEENTH CRUCIFER GENETICS WORKSHOP AND FOURTH ISHS SYMPOSIUM ON BRASSICAS

ISSN： 0567-7572

年卷期： 2006 年 706 期

页码：

收录情况： SCI

摘要： In the genus Raphanus genetic resistance is present against the beet cyst nematode (Heterodera schachtii). Transfer of nematode resistance to rapeseed is desirable to convert this tolerant host plant into a resistant crop. Nematode-resistant winter rapeseed would be of interest as an agronomically important trap crop in rotations with sugar beet. Starting with the initial crosses oil radish x cabbage (RRRR x CCCC) and fodder radish x chinese cabbage (RRRR x AA), a trigenomic hybrid (RRAC) was developed and crossed several times with oilseed rape (AACC). This hybridization programme resulted in a basic population in which the presence of the nine individual radish chromosomes, a to i, was detected by cytogenetic and molecular methods (Peterka et al., 2004). The nematode resistance was localized to chromosome d. After a further cross to rape, we were able to identify for each radish chromosome monosomic addition lines (MAL's) having 38 chromosomes of oilseed rape and one individual chromosome of radish (2n = 4x = 38 + 1; MAL a to i). The added chromosomes in the nine MAL's were cytologically detected with fluorescence in situ hybridization (FISH) using a radish-specific probe. The MAL d was crossed with rape. The progenies were classified with chromosome-specific RAPD/double-primer RAPD markers for presence/absence of the extra chromosome. They were inoculated with L2 juveniles of Heterodera schachtii and cultivated under controlled conditions. The MAL of chromosome d had the same resistance level as the radish variety used as chromosome donor whereas the plants without the radish chromosome were as susceptible as the rapeseed recipient.