文献类型： 外文期刊

作者： Lee, BC ¹ ; He, YK ² ; Murao, K ² ; Isogai, M ² ; Dahal, G ² ; Uyeda, I ² ;

作者机构： 1.YUNNAN ACAD AGR SCI,BIOTECHNOL RES INST,KUNMING,YUNNAN,PEOPLES R CHINA

2.YUNNAN ACAD AGR SCI,BIOTECHNOL RES INST,KUNMING,YUNNAN,PEOPLES R CHINA; INST AGR & ANIM SCI,CHITAWAN,NEPAL

关键词： nucleic acid hybridization;phylogenetic tree analysis;rice dwarf virus

期刊名称：EUROPEAN JOURNAL OF PLANT PATHOLOGY （ 影响因子：1.907； 五年影响因子：2.022 ）

ISSN： 0929-1873

年卷期： 1997 年 103 卷 6 期

页码：

收录情况： SCI

摘要： Rice dwarf virus isolates were collected from several locations in Japan, the Philippines, China, Nepal and Korea. Genomic dsRNA segment profiles in polyacrylamide gel electrophoresis differed among the isolates. There were less differences in the profiles between isolates from Japan and Korea than in those between these two Countries and others. Nucleic acid hybridization was used to examine the extent of genomic variation. Full-length cDNAs to all genomic segments encoding non-structural proteins (S4, S6, S9, S10, S11 and S12) were synthesized from two Japanese isolates, and were used for dot-blot hybridization. Hybridizations using probes generated from the full-length cDNA clones failed to differentiate isolates from different geographical areas. However, cDNA probes covering a variable region of S12 were able to distinguish Japanese and Korean isolates from those of other countries. Phylogenetic tree analysis based on the amino acid sequence of P12 encoded by S12 grouped Japanese and Korean isolates together. The Chinese isolates from two different locations (Yunnan and Fujian) were closely related to each other, and were the most distantly related to Japanese and Korean isolates.