文献类型： 外文期刊

作者： Han, Lichao ¹ ; Ji, Xingzhao ² ; Yang, Caixin ³ ; Zhao, Shuo ¹ ; Fan, Shihong ⁴ ; Zhao, Lijun ³ ; Qiu, Xiaotong ¹ ; Yao, Jiang ⁵ ; Liu, Xueping ⁵ ; Li, Fang ⁶ ; Li, Zhenjun ¹ ;

作者机构： 1.Chinese Ctr Dis Control & Prevent, Natl Inst Communicable Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, Beijing 102206, Peoples R China

2.Shandong First Med Univ, Dept Pulm & Crit Care Med, Shandong Prov Hosp, Jinan 250021, Peoples R China

3.Shanxi Med Univ, Sch Publ Hlth, Dept Epidemiol, Taiyuan 030001, Peoples R China

4.Yunnan Acad Agr Sci, Sericulture & Apiculture Res Inst, Mengzi 661100, Peoples R China

5.Wenzhou Med Univ, Sch Lab Med & Life Sci, Dept Lab, Wenzhou 325035, Peoples R China

6.Tibet Univ, Dept Med, Lhasa 850001, Peoples R China

关键词： Nocardia farcinica; NFA49590; antigenicity; immunoprotection; vaccine

期刊名称：PATHOGENS （ 影响因子：4.531； 五年影响因子：4.58 ）

ISSN：

年卷期： 2022 年 11 卷 12 期

页码：

收录情况： SCI

摘要： Nocardia is emerging as a serious and easily neglected pathogen in clinical practice with multidrug resistance that extends the treatment period for months or even years. This has led to the investigation of a vaccine approach to prevent Nocardia infections. However, studies on the protective proteins of Nocardia have not yet been carried out. In the present work, over 500 proteins in the supernatant of N. farcinica IFM10152 were identified by LC-MS/MS. In silico analysis of these proteins with a high content (score > 2000) predicted that NFA49590 was one of the conserved proteins in N. farcinica strains with potential antigenicity. After the rNFA49590 protein was cloned and expressed in E. coli (DE3) and purified using a Ni-NTA column, its good antigenicity was confirmed with sera from mice immunized with different Nocardia species by Western blot. Then we confirmed its ability to activate innate immunity by examining the phosphorylation status of ERK1/2, JNK, p38, and p65 and the cytokine levels of IL-6, TNF-alpha, and IL-10. Finally, we evaluated its immunoprotective effect in BALB/c mice, and we found that mice immunized with rNFA49590 protein exhibited high antibody titers, enhanced bacterial clearance ability, and generated robust protective effects from the N. farcinica challenge. These results offer strong support for the use of NFA49590 protein as a vaccine candidate and open the possibilities for the exploration of a large array of immunoprotective proteins.