文献类型： 外文期刊

作者： Sun, Peng ¹ ; Zhao, Juan ¹ ; Sha, Gan ¹ ; Zhou, Yaru ¹ ; Zhao, Mengfei ¹ ; Li, Renjian ¹ ; Kong, Xiaojing ¹ ; Sun, Qiping ¹ ; Li, Yun ¹ ; Li, Ke ¹ ; Bi, Ruiqing ¹ ; Yang, Lei ¹ ; Qin, Ziting ¹ ; Huang, Wenzheng ¹ ; Wang, Yin ¹ ; Gao, Jie ² ; Chen, Guang ² ; Zhang, Haifeng ³ ; Adnan, Muhammad ⁴ ; Yang, Long ¹ ; Zheng, Lu ¹ ; Chen, Xiao-Lin ¹ ; Wang, Guanghui ⁵ ; Ishikawa, Toshiki ⁶ ; Li, Qiang ² ; Xu, Jin-Rong ⁷ ; Li, Guotian ¹ ;

作者机构： 1.Huazhong Agr Univ, Ctr Crop Nanotechnol, Hubei Key Lab Plant Pathol, Natl Key Lab Agr Microbiol,Hubei Hongshan Lab, Wuhan, Peoples R China

2.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan, Peoples R China

3.Nanjing Agr Univ, Coll Plant Protect, Key Lab Integrated Management Crop Dis & Pests, Dept Plant Pathol,Minist Educ, Nanjing, Peoples R China

4.Yunnan Acad Agr Sci, Inst Food Crops, Kunming, Peoples R China

5.Northwest A&F Univ, Coll Plant Protect, State Key Lab Crop Stress Biol Arid Areas, Yangling, Peoples R China

6.Saitama Univ, Grad Sch Sci & Engn, Saitama, Japan

7.Purdue Univ, Dept Bot & Plant Pathol, W Lafayette, IN USA

8.Chengdu Normal Univ, Coll Chem & Life Sci, Sichuan Prov Key Lab Dev & Utilizat Characterist H, Chengdu, Peoples R China

9.South China Agr Univ, Natl Key Lab Green Pesticide, Guangdong Prov Key Lab Microbial Signals & Dis Con, Guangzhou, Peoples R China

关键词： alexidine dihydrochloride; lipid metabolism; mitophagy; pathogenesis; Pyricularia oryzae

期刊名称：PLANT CELL AND ENVIRONMENT （ 影响因子：6.0； 五年影响因子：7.6 ）

ISSN： 0140-7791

年卷期： 2024 年

页码：

收录情况： SCI

摘要： Plant pathogens cause devastating diseases, leading to serious losses to agriculture. Mechanistic understanding of pathogenesis of plant pathogens lays the foundation for the development of fungicides for disease control. Mitophagy, a specific form of autophagy, is important for fungal virulence. The role of cardiolipin, mitochondrial signature phospholipid, in mitophagy and pathogenesis is largely unknown in plant pathogenic fungi. The functions of enzymes involved in cardiolipin biosynthesis and relevant inhibitors were assessed using a set of assays, including genetic deletion, plant infection, lipidomics, chemical-protein interaction, chemical inhibition, and field trials. Our results showed that the cardiolipin biosynthesis-related gene MoGEP4 of the rice blast fungus Magnaporthe oryzae regulates growth, conidiation, cardiolipin biosynthesis, and virulence. Mechanistically, MoGep4 regulated mitophagy and Mps1-MAPK phosphorylation, which are required for virulence. Chemical alexidine dihydrochloride (AXD) inhibited the enzyme activity of MoGep4, cardiolipin biosynthesis and mitophagy. Importantly, AXD efficiently inhibited the growth of 10 plant pathogens and controlled rice blast and Fusarium head blight in the field. Our study demonstrated that MoGep4 regulates mitophagy, Mps1 phosphorylation and pathogenesis in M. oryzae. In addition, we found that the MoGep4 inhibitor, AXD, displays broad-spectrum antifungal activity and is a promising candidate for fungicide development.