文献类型: 外文期刊
作者: Li, Jing 1 ; Chen, Si 1 ; Qiu, Run-Shuang 2 ; Zhang, Li-Zhen 2 ; Chen, Yue 2 ; Zheng, Xue 2 ; Li, Ting-Ting 2 ; Zhao, Li-Hua 2 ; Zhang, Zhong-Kai 2 ;
作者机构: 1.Southwest Forestry Univ, Life Sci Coll, Kunming, Yunnan, Peoples R China
2.Yunnan Acad Agr Sci, Yunnan Prov Key Lab Agr Biotechnol, Key Lab Southwestern Crop Gene Resources & Germpl, Minist Agr,Inst Biotechnol & Germplasm Resources, Kunming, Yunnan, Peoples R China
期刊名称:PLOS ONE ( 影响因子:3.752; 五年影响因子:4.069 )
ISSN: 1932-6203
年卷期: 2022 年 17 卷 1 期
页码:
收录情况: SCI
摘要: Tomato zonate spot virus (TZSV), a member of the genus orthotospovirus, causes severe damage to vegetables and ornamental crops in southwest China. The NSs protein is an RNA silencing suppressor in various orthotospovirus like TZSV, but its mechanism and role in virus infection are poorly understood. Here, we observed that an NSs-GFP fusion protein was transiently expressed on the plasma membrane and Golgi bodies in Nicotiana benthamiana plants. The TZSV NSs gene was silenced and infiltrated into N. benthamiana and N. tabacum cv. K326. RT-qPCR and Indirect enzyme-linked immunosorbent assay (ID-ELISA) showed that the transcription and the protein expression of the NSs gene were inhibited by more than 90.00%, and the symptoms on silenced plants were alleviated. We also found that the expression of the Zingipain-2-like gene significantly decreased when the NSs gene was silenced, resulting in co-localization of the NSs-GFP and the Zingipain-2-like-mCherry fusion protein. The findings of this study provide new insights into the mechanism of silencing suppression by NSs, as well as its effect on systemic virus infection, and also support the theory of disease resistance breeding and control and prevention of TZSV in the field.
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