文献类型： 会议论文

第一作者： QU Suping

作者： QU Suping ¹ ; XIONG Li ² ; LI Shufa ² ; WANG Lihua ¹ ; LAND Kaixue ¹ ;

作者机构： 1.Yunnan Academy of Agricultural Sciences, Supervision and Testing Centre for Flowers, Ministry of Agriculture, Kunming 650205, Yunnan, China

2.Flower Research institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan, China

关键词： Roscoea cautheoides;leaf explants;callus;regeneration system

会议名称： The proceedings of the China Association for Science and Technology. Vol. 4, No. 1 /

主办单位：

页码： 548-550

摘要： The ideal regeneration system of Yunnan wild Roscoea cautleoides by leaves explants was established. In this study, the effects of different place on leaf, plant growth regulators, dark periods, casein hydrolysate, coconut milk on callus induction and shoot regeneration were investigated. The research results indicted that downside from leaf, dark periods, casein hydrolysate, coconut milk can improve on callus induction. At the same time, dark periods can facilitate shoot regeneration. The appropriate media for callus induction from leaves explants of Roscoea cautleoides is MS+6-BA1,5mg T1 +NAA0..1mgT' +CH800mgT' +CM50mlT', The appropriate media for shoot regeneration is MS+6-BA1 5mgT1 +NAA0 lmgl"1, The media for propagation adventitious shoots is MS +6-BA06 mg L"1 +NAA0.1 mg-L"', The media for regeneration adventitious roots is 1/2MS +NAA0.8 mg L.

分类号： N5