Functional characterisation of the EgPAL5 promoter from oil palm (Elaeis guineensis Jacq.) reveals its potential roles in plant growth and adaptation to drought
文献类型： 外文期刊
作者： Yusuf, Chong Yu Lok 1 ; Nawawi, Omar 1 ; Azhari, Mohd Farhan 1 ; Hilman, Mohamad Shafek 1 ; Bai, Tianqi 2 ; Zhang, Cuixian 2 ; Yi, Huaifeng 2 ; Ni, Zhangguang 2 ; Xie, Dehong 2 ; Abdullah, Meilina Ong 3 ; Masani, Mat Yunus Abdul 3 ; Shaharuddin, Noor Azmi 4 ; Abdullah, Mohd Puad 5 ; Ong Abdullah, Janna 5 ;
作者机构： 1.Univ Teknol MARA, Fac Plantat & Agrotechnol, Melaka, Malaysia
2.Yunnan Acad Agr Sci, Inst Trop & Subtrop Cash Crops, Baoshan 678000, Yunnan, Peoples R China
3.Malaysian Palm Oil Board MPOB, 6 Persiaran Inst, Kajang, Selangor, Malaysia
4.Univ Putra Malaysia, Fac Biotechnol & Biomol Sci, Dept Biochem, Serdang, Selangor, Malaysia
5.Univ Putra Malaysia, Fac Biotechnol & Biomol Sci, Dept Cell & Mol Biol, Serdang, Selangor, Malaysia
关键词：
Oil palm; phenylalanine ammonia-lyase;
期刊名称：JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY （ 影响因子：2.1； 五年影响因子：2.4 ）
ISSN： 1462-0316
年卷期： 2025 年
页码：
收录情况： SCI
摘要： Oil palm (Elaeis guineensis) is a globally important oil crop whose genome contains five phenylalanine ammonia lyase (EgPAL) genes. In this study, we characterised the regulatory architecture of the EgPAL5 promoter by generating transgenic plants carrying the EgPAL5 promoter fused to GUS. We first conducted an in silico analysis of the 2.3 kb 5' flanking region, identifying multiple cis-regulatory motifs involved in monolignol biosynthesis, osmotic stress response, and hormone signalling. Reporter assays under normal growth conditions revealed broad promoter activity throughout the seedling and adult plant, with developmental modulation manifested as higher GUS expression detected in expanding leaves and developing siliques. Under simulated drought stress, GUS activity increased markedly, indicating strong promoter induction. Moreover, overexpression of the Arabidopsis AtMYB58 transcription factor significantly enhanced EgPAL5 promoter activity, further implying that the EgPAL5 promoter was responsive to regulatory cues associated with lignin biosynthesis. Promoter deletion mapping localised essential cis-elements required for full transcriptional activity to the -1033 to -651 bp region upstream of the transcription start site. Together, these results demonstrate that the EgPAL5 promoter integrates developmental cues and environmental stimuli to modulate phenylpropanoid metabolism.
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