Comprehensive Biochemical, Physiological, and Transcriptomic Analyses Provide Insights Into Floral Bud Dormancy in Rhododendron delavayi Franch
文献类型: 外文期刊
作者: Zhang, Lu 1 ; Song, Jie 1 ; Peng, Lvchun 1 ; Xie, Weijia 1 ; Li, Shifeng 1 ; Wang, Jihua 1 ;
作者机构: 1.Yunnan Acad Agr Sci, Flower Res Inst, Kunming, Peoples R China
2.Natl Engn Res Ctr Ornamental Hort, Kunming, Peoples R China
关键词: transcriptomics; dormancy; Rhododendron delavayi; physiological indices; phytohormones; WGCNA
期刊名称:FRONTIERS IN GENETICS ( 影响因子:4.772; 五年影响因子:4.933 )
ISSN:
年卷期: 2022 年 13 卷
页码:
收录情况: SCI
摘要: Due to a scarcity of relevant data, the ornamental woody flower Rhododendron delavayi Franch. is examined in the current study for its low temperature-induced floral bud dormancy (late October-end December) aspect. This study used transcriptome data profiling and co-expression network analyses to identify the interplay between endogenous hormones and bud dormancy phases such as pre-dormancy, para-dormancy, endo-dormancy, eco-dormancy, and dormancy release. The biochemical and physiological assays revealed the significance of the abundance of phytohormones (abscisic acid, auxin, zeatin, and gibberellins), carbohydrate metabolism, oxidative species, and proteins (soluble proteins, proline, and malondialdehyde) in the regulatory mechanism of floral bud dormancy. The transcriptome sequencing generated 65,531 transcripts, out of which 504, 514, 307, and 240 expressed transcripts were mapped uniquely to pre-, para-, endo-, and eco-phases of dormancy, showing their roles in the stimulation of dormancy. The transcripts related to LEA29, PGM, SAUR family, RPL9e, ATRX, FLOWERING LOCUS T, SERK1, ABFs, ASR2, and GID1 were identified as potential structural genes involved in floral bud dormancy. The transcription factors, including Zinc fingers, CAD, MADS-box family, MYB, and MYC2, revealed their potential regulatory roles concerning floral bud dormancy. The gene co-expression analysis highlighted essential hub genes involved in cold stress adaptations encoding proteins, viz, SERPIN, HMA, PMEI, LEA_2, TRX, PSBT, and AMAT. We exposed the connection among low temperature-induced dormancy in floral buds, differentially expressed genes, and hub genes via strict screening steps to escalate the confidence in selected genes as being truly putative in the pathways regulating bud dormancy mechanism. The identified candidate genes may prove worthy of further in-depth studies on molecular mechanisms involved in floral bud dormancy of Rhododendron species.
- 相关文献
作者其他论文 更多>>
-
Microscopic observation and transcriptome analysis provide insights into mechanisms of hybrid incompatibility in Rhododendron
作者:Xie, Weijia;Li, Shifeng;Peng, Lvchun;Wang, Jihua;Zhang, Lu;Song, Jie;Huang, Hui;Oba, Elias G.
关键词:Rhododendron; Interspecific hybridization; Intraspecific hybridization; Microscopic observation; Transcriptome analysis
-
Jasmonate/ethylene- and NaWRKY6/3-regulated Alternaria resistance depends on ethylene response factor 1B-like in Nicotiana attenuata
作者:Ma, Lan;Duan, Qing;Du, Wenwen;Li, Xiang;Jia, Wenjie;Cui, Guangfen;Wang, Jihua;Ma, Lan;Song, Na;Wu, Jinsong
关键词:Alternaria; ERF; ethylene; jasmonate; Nicotiana; phytoalexin; scopoletin; solavetivone; WRKY
-
Phosphorylated transcription factor PuHB40 mediates ROS-dependent anthocyanin biosynthesis in pear exposed to high light
作者:Zhang, Lu;Wang, Lu;Fang, Yongchen;Gao, Yuhao;Yang, Shulin;Ni, Junbei;Teng, Yuanwen;Bai, Songling;Su, Jun;Teng, Yuanwen
关键词:
-
Evolution of the biosynthetic pathways of terpene scent compounds in roses
作者:Shang, Junzhong;Liu, Heng;Niu, Lintao;Li, Runhui;Li, Yajun;Chen, Mengxi;Li, Ao;He, Yanhong;Bao, Manzhu;Ning, Guogui;Shang, Junzhong;Liu, Heng;Niu, Lintao;Li, Runhui;Li, Yajun;Chen, Mengxi;Li, Ao;He, Yanhong;Bao, Manzhu;Ning, Guogui;Feng, Dedang;Jian, Hongying;Tang, Kaixue;Wang, Jihua;Yan, Huijun;Liu, Zhenhua;Gao, Xiang;Wang, Changquan;Wang, Changquan;Yang, Shuhua
关键词:
-
Heterologous Expression of Platycodon grandiflorus PgF3′5′H Modifies Flower Color Pigmentation in Tobacco
作者:Ma, Lulin;Jia, Wenjie;Duan, Qing;Du, Wenwen;Li, Xiang;Cui, Guangfen;Wang, Xiangning;Wang, Jihua
关键词:PgF3'5'H; transgenic tobacco; over-expression; delphinidin-based anthocyanin; blue flower color
-
Genome-Wide Identification of the RR Gene Family and Its Expression Analysis in Response to TDZ Induction in Rhododendron delavayi
作者:Peng, Lvchun;Zhao, Zhengxiong;Peng, Lvchun;Xie, Weijia;Zhang, Lu;Song, Jie;Li, Shifeng;Li, Xuejiao;Gao, Yan;Zhao, Zhengxiong
关键词:Rhododendron delavayi; adventitious bud regeneration; RR gene family; segmental replication
-
Evolutionary history of two evergreen Rhododendron species as revealed by chromosome-level genome assembly
作者:Wu, Xiaopei;Jin, Guihua;Hu, Yanting;Yang, Hong;Wu, Zhenzhen;Zhang, Chengjun;Wu, Xiaopei;Wu, Zhenzhen;Zhang, Lu;Wang, Jihua;Wang, Xiuyun;Zhang, Rengang;Ma, Yongpeng;Zhang, Rengang;Ma, Yongpeng;Yang, Hong;Zhang, Chengjun
关键词:Rhododendron delavayi; Rhododendron irroratum; genome evolution; gene duplication; anthocyanin biosynthesis; NBS-encoding genes