Identification and characterization of novel antioxidant peptides from Yunnan dry-cured beef: A combined in silico and in vitro study
文献类型： 外文期刊
作者： Wang, Daodian 1 ; Xu, Ziqi 1 ; Wang, Yuzhu 1 ; Li, Yufang 1 ; Zheng, Wentao 1 ; Chai, Yunmei 1 ; Wei, Guangqiang 1 ; Huang, Aixiang 1 ;
作者机构： 1.Yunnan Agr Univ, Coll Food Sci & Technol, Kunming 650201, Yunnan, Peoples R China
2.Yunnan Acad Agr Sci, Sericulture & Apiculture Res Inst, Mengzi 661101, Yunnan, Peoples R China
关键词： Yunnan dry-cured beef; Peptidomics; Antioxidant activity; Stability; Antioxidant peptide; Molecular docking
期刊名称：FOOD CHEMISTRY （ 影响因子：9.8； 五年影响因子：9.7 ）
ISSN： 0308-8146
年卷期： 2025 年 477 卷
页码：
收录情况： SCI
摘要： Dry-cured meats are a good natural source of bioactive peptides. However, there is limited information on the composition and antioxidant activity of peptides in Yunnan dry-cured beef (YDB). This study aimed to identify novel antioxidant peptides from YDB using peptidomics, in silico analysis, and in vitro experimental validation while predicting their antioxidant mechanism through molecular docking. A total of 541 peptides were identified in YDB, with the predominant sources being creatine kinase (13.5 %), myosin (10.4 %), and actin (7.4 %). The novel antioxidant peptides VGSYEDPYH (VH9) and FGEAAPYLRK (FK10) demonstrated a high safety profile, with a hemolysis rate of less than 5 %. Notably, VH9 exhibited excellent ABTS radical scavenging activity (IC50 = 19.698 mu M), DPPH radical scavenging activity (IC50 = 1500.825 mu M), and protection against oxidative stress injury in HepG2 cells. Molecular docking studies revealed that hydrogen bonding and hydrophobic interactions were the primary forces driving the binding of VH9 to the active sites of ABTS, DPPH, Keap1, and myeloperoxidase (MPO). VH9 may protect cells from oxidative damage through radical scavenging, inhibition of reactive oxygen species (ROS) generation, and modulation of the Keap1-Nrf2 antioxidant pathway. Peptides derived from YDB exhibited strong antioxidant activity and showed potential for application as natural antioxidants.
- 相关文献
作者其他论文 更多>>
-
Probiotic potential and safety properties of Limosilactobacillus fermentum A51 with high exopolysaccharide production
作者：Wei, Guangqiang;Wang, Daodian;Wang, Teng;Chai, Yunmei;Li, Yufang;Mei, Minhui;Wang, Hao;Huang, Aixiang;Wang, Daodian;Wang, Gao
关键词：
Limosilactobacillus fermentum A51; whole genome sequencing; probiotic properties; phenotype analysis; safety -
Novel exopolysaccharide from Limosilactobacillus fermentum A10: Biosynthesis, physicochemical properties and antioxidant activity
作者：Wei, Guangqiang;Zhang, Wenbin;Li, Yufang;Wang, Daodian;Chai, Yunmei;Wang, Yuzhu;Xu, Ziqi;Hu, Shaomei;Shi, Jindou;Huang, Aixiang;Zhao, Xingwen;Wang, Daodian
关键词：Exopolysaccharide; Physicochemical properties; Antioxidant activity
-
Identification and molecular mechanism of novel antioxidant peptides from milk tofu cheese: Peptidomics, molecular dynamics, and cell experiments
作者：Wang, Yuzhu;Xu, Ziqi;Wang, Daodian;Zheng, Wentao;Li, Yufang;Wang, Yue;Li, Zhenzhu;Wei, Guangqiang;Huang, Aixiang;Wang, Daodian
关键词：Milk tofu cheese; Antioxidant peptides; Molecular dynamics simulation; Keap1-Nrf2 pathway; Cytoprotective ability
-
Milk-clotting properties on bovine caseins of a novel cysteine peptidase from germinated Moringa oleifera seeds
作者：Wang, Xuefeng;Zhao, Qiong;He, Li;Shi, Yanan;Fan, Jiangping;Huang, Aixiang;Chen, Yue
关键词：germinated Moringa oleifera seed; cysteine peptidase; ?-casein; cleavage site; flocculation reaction
-
Protein function analysis of germinated Moringa oleifera seeds, and purification and characterization of their milk-clotting peptidase
作者：Wang, Xuefeng;He, Li;Zhao, Qiong;Chen, Haoran;Shi, Yanan;Fan, Jiangping;Huang, Aixiang;Chen, Yue
关键词：Germinated Moringa oleifera seeds; Proteomics; Protein function; Milk-clotting activity; Cysteine peptidase
-
Structural Analysis of a Novel Aspartic-Type Endopeptidase from Moringa oleifera Seeds and Its Milk-Clotting Properties
作者：Wang, Xuefeng;He, Li;Zhao, Qiong;Shi, Yanan;Huang, Aixiang;Chen, Yue
关键词：Moringa oleifera seeds; aspartic-type endopeptidase; kappa-casein; cleavage site; flocculation reaction
-
Comparative proteome analysis of matured dry and germinating Moringa oleifera seeds provides insights into protease activity during germination
作者：Wang, Xuefeng;Shi, Yanan;Fan, Jiangping;Pu, Yuehong;Huang, Aixiang;Chen, Yue
关键词：Moringa oleifera seeds; Germination; Proteomics; LC-MS/MS; Protease; Milk-clotting activity
