Milk-clotting properties on bovine caseins of a novel cysteine peptidase from germinated Moringa oleifera seeds
文献类型： 外文期刊
作者： Wang, Xuefeng 1 ; Zhao, Qiong 1 ; He, Li 1 ; Shi, Yanan 1 ; Fan, Jiangping 1 ; Chen, Yue 2 ; Huang, Aixiang 1 ;
作者机构： 1.Yunnan Agr Univ, Coll Food Sci & Technol, Kunming 650201, Yunnan, Peoples R China
2.Yunnan Acad Agr Sci, Biotechnol & Germplasm Resources Inst, Kunming 650205, Yunnan, Peoples R China
关键词： germinated Moringa oleifera seed; cysteine peptidase; ?-casein; cleavage site; flocculation reaction
期刊名称：JOURNAL OF DAIRY SCIENCE （ 影响因子：4.225； 五年影响因子：4.987 ）
ISSN： 0022-0302
年卷期： 2022 年 105 卷 5 期
页码：
收录情况： SCI
摘要： A cysteine peptidase was previously identified from germinated Moringa oleifera seeds, but its milk-clotting properties on bovine caseins was still unclear. In this study, this novel cysteine peptidase (MoCP) showed preferential activity on kappa-casein (kappa-CN), with greater hydrolytic activity compared with calf rennet, whereas weak hydrolysis of alpha-casein and beta-casein made MoCP suitable for application in cheesemaking and may yield various functional peptides. All 3 evaluated caseins were hydrolyzed to form relatively stable peptide bands within 3 h of proteolysis with MoCP. Cleavage sites were determined by gel electrophoresis, liquid chromatography mass spectrometry/mass spectrometry, and peptide sequencing, which revealed that cleavage of kappa-CN by MoCP occurred at residue Ile129-Pro130 and generated a 14,895.37-Da peptide. The flocculation reaction between MoCP and kappa-CN determined by 3-dimensional microscopy with super-depth of field revealed that the initial 30 min of reaction were key for milk coagulation, which may affect curd yield. Overall, the findings presented herein suggest that the cysteine peptidase from germinated M. oleifera seeds can be considered a promising plant-derived rennet alternative for use in cheese manufacture.
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