An antifouling electrochemical aptasensor based on a Y-shaped peptide for tetracycline detection in milk
文献类型： 外文期刊
作者： Pu, Xujun 1 ; Hu, Yuanling 1 ; Niu, Meirong 1 ; Liu, Hongcheng 3 ; Li, Chenguo 2 ; Ma, Wenlong 2 ; Gu, Ying 1 ;
作者机构： 1.Kunming Univ Sci & Technol, Fac Food Sci & Engn, Kunming 650500, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
3.Yunnan Acad Agr Sci, Qual Stand & Testing Technol Res Inst, Kunming 650205, Peoples R China
4.China Univ Petr, Coll Mech & Elect Engn, Qingdao 266580, Peoples R China
关键词： Antifouling; Peptide; Electrochemical aptasensor; Tetracycline; Protein; Food safety
期刊名称：JOURNAL OF FOOD COMPOSITION AND ANALYSIS （ 影响因子：4.0； 五年影响因子：4.1 ）
ISSN： 0889-1575
年卷期： 2024 年 132 卷
页码：
收录情况： SCI
摘要： Nonspecific adsorption of non-target components (especially proteins) in food matrix on sensing surfaces deteriorates accuracy and sensitivity of electrochemical sensors, which is a key challenge for food safety sensing. Therefore, electrochemical sensors with antifouling capability of high-protein food matrices are highly desired. In this paper, an efficient and simple antifouling electrochemical aptasensor for tetracycline (TC) analysis was constructed based on a self-designed Y-shaped peptide [CPPPPEK-(RSERSERSE)2] consisting of cysteine as the anchoring segment, polyproline as the supporting segment, and -EK-(RSERSERSE)2 as antifouling branches. Hydrophilic and electrically neutral amino acid sequence and Y-shaped space structure of the peptide endow the sensing surface with good antifouling performance in protein solutions and diluted milk. Under optimized experiment conditions, the proposed aptasensor can detect TC with a wide linear range (0.01-100 ng mL-1) and a limit of detection of 5.3 pg mL-1 (S/N = 3). The spiked recovery experiments confirmed high accuracy of the aptasensor in diluted milk without other pretreatments (recoveries: 90.96%-95.86%). This strategy offers a valid way to reduce matrix interference, showing a promise to be extended to other sensing systems for different targets.
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