An antifouling electrochemical aptasensor based on a Y-shaped peptide for tetracycline detection in milk
文献类型： 外文期刊
作者： Pu, Xujun 1 ; Hu, Yuanling 1 ; Niu, Meirong 1 ; Liu, Hongcheng 3 ; Li, Chenguo 2 ; Ma, Wenlong 2 ; Gu, Ying 1 ;
作者机构： 1.Kunming Univ Sci & Technol, Fac Food Sci & Engn, Kunming 650500, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
3.Yunnan Acad Agr Sci, Qual Stand & Testing Technol Res Inst, Kunming 650205, Peoples R China
4.China Univ Petr, Coll Mech & Elect Engn, Qingdao 266580, Peoples R China
关键词： Antifouling; Peptide; Electrochemical aptasensor; Tetracycline; Protein; Food safety
期刊名称：JOURNAL OF FOOD COMPOSITION AND ANALYSIS （ 影响因子：4.0； 五年影响因子：4.1 ）
ISSN： 0889-1575
年卷期： 2024 年 132 卷
页码：
收录情况： SCI
摘要： Nonspecific adsorption of non-target components (especially proteins) in food matrix on sensing surfaces deteriorates accuracy and sensitivity of electrochemical sensors, which is a key challenge for food safety sensing. Therefore, electrochemical sensors with antifouling capability of high-protein food matrices are highly desired. In this paper, an efficient and simple antifouling electrochemical aptasensor for tetracycline (TC) analysis was constructed based on a self-designed Y-shaped peptide [CPPPPEK-(RSERSERSE)2] consisting of cysteine as the anchoring segment, polyproline as the supporting segment, and -EK-(RSERSERSE)2 as antifouling branches. Hydrophilic and electrically neutral amino acid sequence and Y-shaped space structure of the peptide endow the sensing surface with good antifouling performance in protein solutions and diluted milk. Under optimized experiment conditions, the proposed aptasensor can detect TC with a wide linear range (0.01-100 ng mL-1) and a limit of detection of 5.3 pg mL-1 (S/N = 3). The spiked recovery experiments confirmed high accuracy of the aptasensor in diluted milk without other pretreatments (recoveries: 90.96%-95.86%). This strategy offers a valid way to reduce matrix interference, showing a promise to be extended to other sensing systems for different targets.
- 相关文献
作者其他论文 更多>>
-
Quantification of caffeine and catechins and evaluation of bitterness and astringency of Pu-erh ripen tea based on portable near-infrared spectroscopy
作者：Wu, Zhongdong;Li, Chenghuan;Yi, Lunzhao;Ren, Dabing;Gu, Ying;Wang, Shuo;Liu, Hongcheng;Lin, Tao
关键词：Near-infrared spectroscopy; Pu-erh ripen tea; Caffeine; Catechins; Bitterness; Astringency
-
Traceability Research on Geographic Erigeron breviscapus Based on High-Resolution Mass Spectrometry and Chemometric Analysis
作者：Zhang, Jiao;Tian, Heng;Lin, Tao;Liu, Hongcheng;Zhang, Jiao;Huang, Xiangzhong;Tian, Heng;Tian, Heng
关键词：Erigeron breviscapus; geographic origin; chemometric analysis
-
Traceability Research on Dendrobium devonianum Based on SWATHtoMRM
作者：Lin, Tao;Chen, Xinglian;Du, Lijuan;Liu, Zhenhuan;Liu, Hongcheng;Wang, Jing;Hu, Zhengxu;Cheng, Long
关键词：time-of-flight mass spectrometry; identification; Dendrobium devonianum; Longling area
-
Metabolite of chiral cycloxaprid in solvent and in the raw of Puer tea
作者：Tian, Hen;Lin, Tao;Li, Qiwan;Liu, Hongcheng;Tian, Hen;Zhang, Jiao;Huang, Xiangzhong;Liu, Hongcheng
关键词：Metabolite; Cycloxaprid; Raw Puer tea processing
-
The evaluation of the nutritional quality of common wild edible fungi in Yunnan Province
作者：Du, Lijuan;Ye, Yanping;Lin, Tao;Li, Maoxuan;Bi, Yanan;Li, Qiwan;Liu, Hongcheng;Li, Qiwan;Liu, Hongcheng
关键词：nutritional evaluation; wild edible fungi; Yunnan Province
-
Generic imprinted fiber array strategy for high-throughput and ultrasensitive simultaneous determination of multiple neonicotinoids
作者：Wang, Dan;Liu, Yuanchen;Xu, Zhigang;Ji, Yang;Liu, Zhimin;Si, Xiaoxi;Lin, Tao;Liu, Hongcheng
关键词：Generic imprinted fiber array; Molecular imprinting; High throughput; Enrichment strategy; Neonicotinoid
-
A Review on Molecularly Imprinted Polymers Preparation by Computational Simulation-Aided Methods
作者：Liu, Zhimin;Ma, Liping;Xu, Zhigang;Wang, Dan;Yang, Yuming;Duan, Yunli;Lin, Tao;Liu, Hongcheng
关键词：computational simulation; molecularly imprinted polymers; intermolecular interaction
