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The transcription factor PjERF1 enhances the biosynthesis of triterpenoid saponins in Panax japonicus

文献类型： 外文期刊

作者： Chen, Qin 1 ; Yu, Yilin 1 ; Zhang, Xiang 1 ; Zhao, Ren 3 ; Zhang, Jinyu 4 ; Liu, Diqiu 1 ; Cui, Xiuming 1 ; Ge, Feng 1 ;

作者机构： 1.Kunming Univ Sci & Technol, Fac Life Sci & Technol, Kunming 650500, Yunnan, Peoples R China

2.Kunming Univ Sci & Technol, Key Lab Sustainable Utilizat Panax Notoginseng Re, Kunming 650500, Yunnan, Peoples R China

3.Yunnan Prov Inst Mat Med, Kunming 650111, Yunnan, Peoples R China

4.Yunnan Acad Agr Sci, Inst Med Plants, Kunming 650205, Yunnan, Peoples R China

关键词： Transcription factor; Panax; Saponins; Triterpenoid; Promoter

期刊名称：PLANT BIOTECHNOLOGY REPORTS （ 影响因子：2.01； 五年影响因子：1.907 ）

ISSN： 1863-5466

年卷期： 2021 年 15 卷 5 期

页码：

收录情况： SCI

摘要： The ERF-type transcription factors (TFs) play vital roles in plant secondary metabolism. ERF TFs simultaneously regulate the expression levels of key enzyme genes involved in the biosynthesis of secondary metabolites due to its "multi-point control" function. In this study, one gene of ERF TFs from Panax japonicus (PjERF1) was cloned. The open reading frame of PjERF1 was 801 bp and encoded 266 amino acids. Phylogenetic analysis showed that PjERF1 belonged to ERF subfamily with a typical conserved domain. Subcellular localization found that PjERF1 protein might be located in eukaryotic cell nucleus. Yeast one-hybrid assay demonstrated that PjERF1 could bind to the promoters of Pj beta AS, PjCAS, and PjSE specifically and regulate the expression levels of such key enzyme genes involved in the triterpene saponins biosynthesis. Therefore, in the PjERF1 overexpression cell lines, the expression levels of some key enzyme genes involved in the triterpenoid saponins biosynthesis were significantly increased compared with those in non-transgenic cell line. As a result of it, the biosynthesis of chikusetsusaponin IV and IVa, and other ginsenosides (Rd, Rb1, Re, and R0) were also promoted in the PjERF1 overexpression cell lines. This study indicated that PjERF1 could regulate the biosynthesis of saponins in P. japonicus through controlling the expression levels of key enzyme genes related to the biosynthesis of triterpenoid saponins.

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